不同植物激素对黄檗叶片愈伤组织诱导效应

以黄檗无菌苗叶片为外植体，研究了生长素NAA、2,4-D、细胞分裂素6-BA以及二类不同植物激素组合对黄檗愈伤组织诱导和生长的影响。结果表明，单独使用6-BA不能诱导出黄檗叶片的愈伤组织，单独使用NAA和2,4-D 对愈伤诱导率不高，最高分别是53.33%和73.33%且愈伤质量差。当生长素与细胞分裂素配合使用时，以BA 1.5 mg/L+ 2,4-D 2.0 mg/L激素组合愈伤组织诱导率高达100%，生长最旺盛。

Effects of Different Phytohormones on Leaf Callus Induction of Phellodendron amurense Rupr.

In order to improve percentage of callus induction from Phellodendron amurense, the axenic leaf of P. amurense was used as explants to study the effect of different combinations of plant hormones on callus induction in P. amurense. The results showed that the callus induction could not be succeeded with 6-BA only. The induction rate was low when sole NAA or 2,4-D was used, and the highest rates of the callus induction were 53.33% and 73.33% , respectively, with poor callus quality. When auxin and cytokinin were applied together, the callus induction was improved, and the optimum phytohormone combination was BA 1.5 mg/L＋ 2,4-D 2.0 mg/L, which induction rate was 100%, and the callus grew luxuriantly.