铁在寒地早粳花药培养中的效应研究

摘要：铁是水稻花培培养基的重要成分。通过对7份寒地早粳组合F1（或品系）花药在不同Fe2+含量培养基上离体培养，发现培养基中的Fe2+的含量对诱导率、绿苗分化率影响较大。结果表明在N6培养基中将Fe2+提高到32-40mg/kg诱导率最大，高于N6培养基（Fe2+含量为5.6mg/kg）1.4倍以上。在此以内随着Fe2+含量的增加诱导率增加，大于此含量诱导率降低，呈单峰曲线分布。分化培养基中Fe2+含量增加到40mg/kg分化率显著降低。但在32-40mg/kgFe2+培养基上诱导出的愈伤组织在MS培养基上的绿苗分化率差异明显，71.4%材料可使绿苗生产率提高到9.2%左右。因此适当提高诱导培养基中Fe2+含量可提高寒地早粳的花培效率。

The iron effect study of early japonica anther culture in cold region

Abstract: Iron is an important component in anther culture medium of rice. In this study, through vitro culturing anthers of 7 early japonica combinations F1 of cool region (lines) in medium of different Fe2+ content , we found that Fe2+ content have greater impact in the induction rate and the rate of green plant on differentiation. The results showed that Fe2+ will be increased to 32-40 mg/kg had largest induced rate in N6 medium, higher than the N6 medium (Fe2+ content is 5.6mg/kg) 1.4 times more, in this within, with Fe2+ content increase, the induced rate increase, more than this content, the induced rate induce, showed a single peak of distribution. When Fe2+ content in differentiation medium increased to 40mg/kg, the differentiation rate was significantlylower. But the rate of green plant on the differentiation of callus was obvious differences in MS medium, which was induced in the medium of 32-40 mg/kg Fe2+ content, about 71.4% of materials can improve the efficiency of anther culture to 9.2%.So, we consider that increasing the Fe2+ content in induced medium can increase anther culture efficiency of early japonica in cold region.