新疆伊犁某牛场不同生长期牛粪源大肠杆菌耐药性调查

为了调查新疆伊犁某牛场不同生长期牛粪源大肠杆菌对临床常用抗菌药物的耐药情况。从该牛场分别采集育肥牛、繁育牛、加育牛和未繁育牛的粪样。采用微量肉汤稀释法,对分离出的大肠杆菌进行最小抑菌浓度测定。结果表明,育肥牛、繁育牛、加育牛和未繁育牛粪源大肠杆菌分离率均为100%,不同生长期的牛源大肠杆菌对氨苄西林均耐药,耐药率分别为4.0%、8.6%、6.7%、20.0%;对安普霉素都较为敏感。繁育牛和加育牛粪源大肠杆菌耐药种类较多,未繁育牛粪源大肠杆菌对比其他生长期牛粪源菌耐药较严重,不同生长期的牛粪源大肠杆菌多重耐药情况不太严重,主要为0耐、1耐;新疆伊犁该牛场对临床常用抗菌药物的耐药程度不同,耐药程度虽然不高,但在养殖生产中需注意合理用药。

Survey of Resistance Escherichia coli Isolates to Antibiotics from Different Growth Stages Cattle in A Cattle Farm in Ili, Xinjiang

The study aims to investigate resistance of Escherichia coli isolates from cow dung at different cattle growth stages to commonly used antibiotics in a cattle farm in Ili, Xinjiang. Fecal samples were taken from fattening cattle, breeding cattle, strengthen fattening cattle and non-breeding cattle. Minimal inhibitory concentrations of the Escherichia coli isolates were determined by the broth micro-dilution method. Results showed that isolation rate of Escherichia coli from fattening cattle, breeding cattle, strengthen fattening cattle and non-breeding cattle were all 100%; Escherichia coli of different cattle growth stages showed resistance to ampicillin, resistance rates were 4.0%, 8.6%, 6.7% and 20.0%, respectively, and were more sensitive to apramycin. Resistant of Escherichia coli isolates of non-breeding cattle was more serious than that of the others. Multi-drug resistance of Escherichia coli isolates from cattle at different growth stages was less serious, and most of the isolates were resistant to zero and one antibiotic. While the level of drug resistance is not very high, rational drug application should be observed in production.