16份薏苡种质苗期耐寒性鉴定

本研究旨在筛选出抗寒性较高的薏苡种质,为薏苡杂交育种和栽培提供重要依据。以16份薏苡种质为试验材料,在3℃低温胁迫下培养3天,测定与抗寒性相关的生理指标,并采用极点排序法进行耐寒性综合评价。结果表明：低温(3℃)胁迫下,叶片叶绿素含量和超氧化物歧化酶(SOD)活性下降,种质叶片电导率含量、丙二醛含量、可溶性糖含量、脯氨酸含量、可溶性蛋白含量不同程度上升;在低温和常温的处理下,这些生理指标的变化有明显的差异,说明其变化与薏苡抗寒性密切相关;通过极点排序法得到薏苡抗寒能力排序：‘云野-6’<‘YGL’<‘云野-10’<‘云野-8’<‘云野-12’<‘云野-1’<‘云野-4’<‘云野-3’<‘云野-5’<‘YLO’<‘YOL-12’<‘云野-7’<‘云野-9’<‘云野-11’<‘云野-2’<‘Y9-5’。16个供试种质中‘Y9-5’的抗寒能力最强,‘云野-2’次之,‘云野-6’最弱,可将‘Y9-5’和‘云野-2’作为薏苡抗寒杂交育种和栽培的良好材料。

Identification of Cold Tolerance in 16 Coix Germplasm

The research aims to screen out the germplasm of Coix with high cold resistance, and provide a basis for hybrid breeding and cultivation of Coix. Taking 16 germplasms of Coix as test materials, we cultured the materials under low temperature stress at 3℃ for 3 days, measured the physiological indexes related to cold resistance, and comprehensively evaluated cold resistance by using a pole-sorting method. The results showed that under low temperature (3℃) stress, the chlorophyll content and SOD activity of leaves decreased, and the electrical conductivity content, malondialdehyde content, soluble sugar content, proline content, and soluble protein content of leaves of various germplasms increased to varying degrees. Under the treatment of low temperature and normal temperature, there were obvious differences in the change of these physiological indicators, indicating that the changes are closely related to the cold resistance of the Coix. The rank of the cold resistance of the Coix obtained by the pole-sorting method is: ‘Yunye-6’<‘YGL’<‘Yunye-10’<‘Yunye-8’<‘Yunye-12’<‘Yunye-1’<‘Yunye-4’<‘Yunye-3’<‘Yunye-5’<‘YLO’<‘YOL-12’<‘Yunye-7’<‘Yunye-9’<‘Yunye-11’<‘Yunye-2’<‘Y9-5’. Among the 16 germplasms, ‘Y9-5’ and ‘Yunye-2’could be used as cold resistance material for hybrid breeding and cultivation.