泛素化修饰及其在植物蛋白质组学中的研究进展

泛素化修饰是真核生物中一种重要的蛋白质翻译后修饰,几乎参与调控细胞内所有重要的生命活动过程。近年来,随着泛素化蛋白质富集技术和质谱技术的不断发展,泛素化蛋白质组学研究取得了重大进展。目前泛素化蛋白质组学研究多集中在动物和酵母中,而在植物中开展的相对较少。为了总结植物中泛素化蛋白质组学的最新研究成果,本文对泛素化修饰、质谱法鉴定泛素化位点、泛素化蛋白的富集方法及泛素化蛋白质组学在植物光调节、激素处理和逆境胁迫条件下的应用研究进行综述,为泛素化修饰在植物领域的最新研究提供理论基础。

Ubiquitin Modification and Its Research Progress in Plant Proteomics

Ubiquitination modification is an important post-translational modification of proteins, which participates in the regulation of many important life activities in cells. In recent years, with the development of ubiquitinated proteins enrichment technology and mass spectrometry technology, great progress has been made in ubiquitination proteomics. At present, there are many ubiquitination proteomics studies in animals and yeasts, but few in plants. In order to summarize the latest research results of ubiquitination proteomics in plants, the ubiquitination modifications, the identification of ubiquitination sites by mass spectrometry, the enrichment methods of ubiquitination proteins and the application of ubiquitination proteomics in plant light regulation, hormone treatment and stress conditions were reviewed in this paper, which could provide the rationale for the latest research of ubiquitination modifications in plants.