不同处理对芹菜种子萌发过程中抗氧化系统及激素水平的影响

为研究芹菜种子的萌发机制,采用不同试剂组合（5 g/L NaOH+10% PEG、10% PEG+500 mg/L壳聚糖、5 g/L NaOH+500 mg/L壳聚糖）和不同温度(18、24、30℃)对芹菜种子进行处理,观察芹菜种子的萌发指标,并探究种子萌发过程中的抗氧化系统及内源激素水平的变化。结果表明,30℃对种子发芽率、发芽势及发芽指数提高具有促进作用,加快种子萌发进程。与蒸馏水对照组相比,试剂组合处理在18、24℃时种子发芽率、发芽势和发芽指数升高,促进种子萌发,18℃时5 g/L NaOH+500 mg/L壳聚糖处理下的发芽势最高,24℃时5 g/L NaOH+10% PEG处理下发芽势达到最大值;30℃时不同试剂组合处理间发芽率、发芽势和发芽指数无明显差异。18、30℃对种子具有一定程度的胁迫作用,种子SOD和CAT活性较24℃条件下有所增加;18℃时MDA含量和脯氨酸含量显著增加;5 g/L NaOH+10% PEG处理能显著降低MDA含量。萌发过程中,芹菜种子内部ABA含量下降,GA₃、ZA含量增加。试剂组合及适当高温有利于提高芹菜种子的发芽率、发芽势和发芽指数,促进种子萌发,提高种子萌发整齐度;芹菜种子萌发过程中抗氧化系统和激素水平会积极响应不同的处理条件。

Effects of Different Treatments on the Antioxidant System and Hormones During the Germination of Celery Seeds

To study the germination mechanism of celery seeds, different reagent combinations (5 g/L NaOH+ 10% PEG, 10% PEG+ 500 mg/L chitosan, 5 g/L NaOH+ 500 mg/L chitosan) and different temperatures (18, 24, 30℃) were used to process the seeds and to explore the germination index, the changes in antioxidant system and endogenous hormone levels in seed germination. The results showed that 30℃ could promote the germination rate, germination potential and germination index of seeds, and accelerate the germination process of seeds. Compared with the control group of distilled water, the germination rate, germination potential and germination index of the combination treatment increased at 18 and 24℃. Under the 18℃, 5 g/L NaOH+ 500 mg/L chitosan treatment, the potential was the highest, and the germination potential reached the maximum under the treatment of 5 g/L NaOH+ 10% PEG at 24℃; there was no significant difference in germination rate, germination potential and germination index between different reagent combinations at 30℃. 18 and 30℃ had a certain degree of stress on seeds, and the activities of SOD and CAT in seeds increased compared with those under 24℃. The content of MDA and proline significantly increased at 18℃. The content of MDA was significantly reduced by the treatment of 5 g/L NaOH+ 10% PEG reagent combination. During the germination, the content of ABA gradually decreased, and the contents of GA₃ and ZA gradually increased in celery seeds. Therefore, the combination of reagents and appropriate high temperature are beneficial to increase the germination rate, germination potential and germination index of celery seeds, and can also improve the germination uniformity. The antioxidant system and hormone levels of celery seeds actively respond to different treatment conditions during the germination process.