重组酶聚合酶等温扩增技术在动物源性成分鉴定中的应用

在打击肉制品掺假走私、维护市场秩序、保护野生动物资源等方面,对动物源性成分鉴定技术的特异性、灵敏性、重复性提出了很高的要求。本文阐述了动物源性成分的检测鉴定技术,有多样性和局限性的特点。归纳了重组酶聚合酶恒等温扩增技术原理与应用,解决了PCR技术在仪器上的局限性,优化了早期恒温扩增技术的缺点。指出重组酶聚合酶恒等温扩增技术为动物源性成分的快速准确鉴定提供了坚实的技术基础,并在动物疫病诊断方面也有很好的应用前景。

Recombinase-Polymerase Mediated Isothermal Amplification Technology: Application in Identification of Animal-Derived Ingredients

The identification technology of animal-derived ingredients requires high specificity, sensitivity and repeatability of detection methods in combating adulteration and smuggling of meat products, maintaining market orders and protecting wildlife resources. In this study, the detection and identification technologies of animal-derived ingredients were described, which had the characteristics of diversity and limitation. The principle and application of recombinase-polymerase mediated isothermal amplification technology were summarized, the limitations of PCR technology on instruments were solved, and the early isothermal amplification technology was optimized. The study points out that the recombinase-polymerase mediated isothermal amplification technology could be a technical basis for the rapid and accurate identification of animal-derived ingredients, and has a good application prospect in the diagnosis of animal epidemics.