降香黄檀愈伤组织培养与植株再生研究

为了实现降香黄檀工厂化快速繁殖和扩大栽培,并为降香黄檀抗寒基因导入打下一定的基础,以降香黄檀无菌实生苗茎段、叶片、根尖为外植体,MS为基本培养基,对各器官愈伤组织诱导与分化的最适培养基成分进行了研究。结果表明,可从降香黄檀无菌实生苗茎段、叶片、根尖成功地进行愈伤组织培养和植株再生。茎段、叶片、根尖诱导愈伤组织的最适培养基分别为1/2MS+6-BA1.5mg/L+NAA0.10mg/L、1/2MS+6-BA0.5mg/L+NAA0.10mg/L和1/4MS+6-BA1.5mg/L+NAA0.05mg/L;茎段、叶片、根尖的愈伤组织诱导丛生芽最适培养基分别为1/2MS+6-BA1.5mg/L+2,4-D4.0mg/L、1/2MS+6-BA1.5mg/L+2,4-D3.5mg/L和1/2MS+6-BA1.5mg/L+2,4-D3.5mg/L;茎段、叶片、根尖来源的单芽,其最佳生根培养基分别为MS+NAA1.5mg/L、MS+NAA1.0mg/L和MS+NAA2.0mg/L。比较茎段、叶片与根尖的愈伤组织诱导率、丛生芽诱导率和单芽生根率,得出以无菌实生苗根尖作为外植体是降香黄檀愈伤组织培养和植株再生的最佳选择。

Callus Culture and Plant Regeneration of Dalbergia odorifera

In order to realize the industrialized rapid propagation and expand cultivation of Dalbergia odorifera and lay a certain foundation for the introduction of cold resistance genes, optimal medium composition for callus induction and differentiation was investigated, with stems, leaves, root apexes of sterile seedlings as explants and MS as basic medium. The results showed that the callus culture and plant regeneration from the various organs were successfully achieved. The optimal media for callus induction from stem segments, leaves, root apexes of sterile seedlings were 1/2MS + 6-BA 1.5 mg/L + NAA 0.10 mg/L, 1/2MS + BA 0.5 mg/L + NAA 0.10 mg/L and 1/4MS+6-BA1.5 mg/L+NAA 0.05 mg/L, respectively. The optimal media for the proliferation of clustered shoots from the callus inducted from the three organs were 1/2MS+6-BA 1.5 mg/L+2,4-D 4.0 mg/L, 1/2MS+6-BA 1.5 mg/L+2,4-D 3.5 mg/L and 1/2MS+6-BA 1.5 mg/L+2,4-D 3.5 mg/L, respectively. The best rooting media for single shoots from the callus of the three sources were MS + NAA 1.5 mg/L, MS + NAA 1.0 mg/L and MS + NAA 2.0 mg/L, respectively. In light of the rates of callus induction, proliferation of clustered shoots and rooting, the best system of callus culture and plant regeneration of Dalbergia odorifera was established with the root apexes of the sterile seedlings as explants.