动物饲用益生菌LPF-2对大肠杆菌抑制作用的培养条件优化

研究目的]对畜禽大肠杆菌具有抑制作用的动物饲用益生菌短短芽孢杆菌LPF-2的培养条件进行优化.方法]以抑菌圈为指标,从时间、温度、摇床转速和培养基pH值研究短短芽孢杆菌LPF-2的最适培养条件,以获得短短芽孢杆菌LPF-2最佳的抑菌效果.结果]短短芽孢杆菌LPF-2培养24～84h的抑菌圈为10.00～13.00 mm,在24～39℃下培养的抑菌圈为9.00～14.00 mm,转速在90～210 r/min下培养的抑菌圈为13.75～15.00 mm,pH 6.0～8.0下培养的抑菌圈为10.50～14.50 mm.结论]动物益生菌短短芽孢杆菌LPF-2的最适培养条件为培养基pH 7.5、培养温度30℃、培养时间48 h,摇床转速在90～210 r/min范围内均可.

Optimization of Fermentation Conditions for Animal Feed Probiotics, Brevibacillus brevis LPF-2, Inhibiting Against Escherichia coli

OBJECTIVE] The fermentation conditions were optimized for animal feed probiotics, Brevibacillus brevis LPF-2, which strongly inhibited Escherichia coli. METHOD] The best fermentation conditions for the highest inhibition of LPF-2 were studied based on time, temperature, shaking speed and medium pH value by using inhibition zones as an index. RESULTS]The results showed that its inhibition zones against E. Coli were 10.00-13.00 mm after cultured for 24-84 h, 9.00-14.00 mm at 24-39 ℃, 13.75-15.00 mm at the shaking speeds of 90-210 r/min and 10.50-14.50 mm at pH values of 6.0-8.0, respectively. CONCLUSION] n conclusion, the best fermentation conditions for LPF-2 against E. Coli were as follows: pH 7.5, 30 ℃, 48 h culture time and 90-210 r/min shaking speed.