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慢病毒介导的shRNA对MFC细胞生长抑素的沉默及对细胞增殖的影响
引用本文:马强,于浩,曹婧华,王楠,刘松财,张永亮,郝林琳.慢病毒介导的shRNA对MFC细胞生长抑素的沉默及对细胞增殖的影响[J].中国农学通报,2011,27(11):50-53.
作者姓名:马强  于浩  曹婧华  王楠  刘松财  张永亮  郝林琳
作者单位:吉林大学农学部;中国兽医药品监察所;华南农业大学动物科学学院;
基金项目:国家自然科学基金“抗体封闭猪垂体SSTR2和5方法的建立及其对猪生长的影响”(30771572)
摘    要:SS能够抑制肿瘤细胞的增殖,其与肿瘤的关系已成为研究热点。将包装获得的靶向生长抑素的lv-shRNA病毒液感染MFC细胞,同时,以pcDNA3.1-SS(pSS)转染细胞组作为SS过表达细胞对照,通过荧光显微镜观察到了绿色荧光蛋白(GFP)的高效表达,RIA检测发现49.55%(P〈0.05)的SS受到了抑制。MTT法绘制细胞的生长曲线可知,pSS转染细胞的生长明显受到抑制,而LV-shRNA组细胞的生长密度比对照组细胞提高了19.27%(P〈0.05),表明SS对肿瘤细胞的增殖具有抑制作用。文章为SS及其类似物在肿瘤方面的治疗提供了理论依据。

关 键 词:生长抑素  MFC细胞  细胞增殖
收稿时间:2010/11/26 0:00:00
修稿时间:2010/12/8 0:00:00

the Effects of Lentiviral-directed shRNA on Somatostatin Silencing and the Cell Proliferation of MFC Cells
Ma Qiang ,Yu Hao ,Cao Jinghua ,Wang Nan ,Liu Songcai ,Zhang Yongliang ,Hao Linlin.the Effects of Lentiviral-directed shRNA on Somatostatin Silencing and the Cell Proliferation of MFC Cells[J].Chinese Agricultural Science Bulletin,2011,27(11):50-53.
Authors:Ma Qiang  Yu Hao  Cao Jinghua  Wang Nan  Liu Songcai  Zhang Yongliang    Hao Linlin
Institution:Ma Qiang 1,Yu Hao 1,Cao Jinghua 1,Wang Nan 3,Liu Songcai 1,Zhang Yongliang 1,2,Hao Linlin 1 (1 Agricultural Division of Jilin University,Jilin University,Changchun 130062,2 College of Animal Science,South China Agricultural University,Guangzhou 510642,3 China Institute of Veterinary Drugs Control,Beijing 100081)
Abstract:Somatostatin inhibits the growth of tumor cells,and the correlation between somatostatin and the tumor is becoming a hot topic.MFC cells were infected with the lv-shRNA viral stocks targeting somatostatin with pcDNA3.1-SS transfected cells as the positive controls.The the fluorescence observation showed that high infection efficiency was gained.SS protein level in LV-shRNA infected cells were reduced 49.55% (P< 0.05) detected with RIA.Cell growth curve measured using the MTT assay showed that high levels of...
Keywords:somatostatin  MFC cell  cell proliferation  
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