EMS诱变谷子‘长农35号’M1代成熟期株型突变体的鉴定与分析

为建立谷子突变体库，以便在谷子分子生物学研究中应用，以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料，采用0.8%和1.0%甲磺酸乙酯(EMS)进行了诱变处理，试验共收获株型相关M1代突变体材料282份，其中，0.8% EMS诱变‘长农35号’获得株型突变体材料100个单株，可分为10个组；1.0% EMS诱变‘长农35号’获得株型突变体材料182个单株，可分为17个组。M1代成熟期株型相关性状突变体分析结果表明：1.0% EMS处理‘长农35号’所获突变体，在株高、穗下节粗、穗下第一节间粗和茎节数4个性状与对照有显著差异，而0.8%处理与对照差异不显著，因此，对于‘长农35号’来讲，采用1.0% EMS进行诱变处理更有利于多类型大量突变单株的获得。

Identification and Analysis of Mature Plant Type Mutants of M1 Generation of ‘Changnong35’, a Foxtail Millet Variety, Treated with EMS Mutagenesis

Foxtail millet cultivar ‘ Changnong35 ＇,which is widely used in agricultural production,was treated with EMS to establish foxtail millet mutant library for the molecular biology applications.We obtained 282 mutants in the M 1 generation related to plant type,of which,100 mutant plants obtained by using 0.8% EMS treatment can be divided into 10 groups;182 mutant plants obtained by using 1.0% EMS treatment can be divided into 17 groups.The analysis results of the mature plant type traits of the M 1 generation showed thatplant height,stem diameter under spike,the diameter of the first internode and internode numbers treated with 1.0% EMS comparing with the control were significantly different,while the 0.8% EMS treatment comparing with control was not significant,therefore,the treatment of 1.0% EMS was more conducive to obtainment of a large number and multi-type mutants for ＇ Changnong35 ＇.