| 双孢蘑菇栽培菌株遗传多样性的DNA指纹分析 |
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| 引用本文： | 陈美元,廖剑华,李洪荣,等.双孢蘑菇栽培菌株遗传多样性的DNA指纹分析[J].中国农学通报,2009,25(4):149-156. |
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| 作者姓名： | 陈美元 廖剑华 李洪荣 等 |
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| 作者单位： | 福建省农业科学院,福建省蘑菇菌种研究推广站,福州,350014 |
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| 基金项目： | 国家自然科学基金，福建省科技厅重大项目，福建省自然科学基金重点项目，福建省农科院创新基金 |
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| 摘 要： | 对双孢蘑菇206个栽培菌株的总DNA进行大规模的SRAP、ISSR和RAPD分析，共获得15条SRAP、3条ISSR和2条RAPD标记。利用这20个标记对206个菌株进行聚类分析，获得亲缘关系树状图。结果显示在28%的相似值上，206个菌株可以分为高产型和优质型两大类群，而在100%相似值上，可分为大小72个类群，每群包含菌株数为1~31不等。
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| 关 键 词： | 双孢蘑菇 SRAP ISSR RAPD 聚类分析 |
| 收稿时间： | 2008-10-30 |
| 修稿时间： | 2009-01-07 |
DNA fingerprinting of genetic diversity of Agaricus bisporus cultivation strains |
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| Chen Mei-yuan,Liao Jian-hua,Li Hong-rong,Guo Zhong-jie,Lu Zheng-hui,Cai Dan-feng,Wang Ze-sheng.DNA fingerprinting of genetic diversity of Agaricus bisporus cultivation strains[J].Chinese Agricultural Science Bulletin,2009,25(4):149-156. |
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| Authors: | Chen Mei-yuan Liao Jian-hua Li Hong-rong Guo Zhong-jie Lu Zheng-hui Cai Dan-feng Wang Ze-sheng |
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| Institution: | Chen Meiyuan;Liao Jianhua;Li Hongrong;Guo Zhongjie;Lu Zhenghui;C ai Danfeng;Wang Zesheng(Fujian Mushroom R＆D Station;Fujian Academy of Agricultural Science;Fuzhou 350014) |
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| Abstract: | 206 cultivation strains of Agaricus bisporus were studied by using the techniques of SRAP, ISSR and RAPD fingerprinting, and 15 special SRAP bands, 3 special ISSR bands and 2 special RAPD bands were obtained. The strains were clustered based on the 20 special bands and a tree map was obtained. The results showed that 206 strains be divided into high-production group and good-quality group at the similarity coefficient of 28%, while at the similarity coefficient of 100%, the strains were divided into 72 groups with strain number range of 1~31. |
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| Keywords: | SRAP ISSR RAPD |
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