甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析

为了进一步研究甜菜谷胱甘肽转移酶BvGSTU9 (LOC104894060)在重金属胁迫过程中的功能。本研究以‘780016B/12优’为实验材料,对该基因序列特征、结构、功能进行预测分析,并利用qPCR检测该基因在不同浓度镉胁迫下的表达量变化。结果显示甜菜BvGSTU9基因全长925 bp,开放阅读框675 bp,编码了由224个氨基酸组成的不稳定膜外蛋白。BvGSTU9与菠菜、藜麦的氨基酸序列相似性较高,与系统发育进化树分析结果基本相符。二级和三级结构预测表明该基因主要由α-螺旋、β-折叠、延伸链及无规则卷曲组成。qPCR显示BvGSTU9基因在不同浓度的镉胁迫下均受到不同程度的诱导,因此可以推断甜菜BvGSTU9基因无论从结构还是功能上,与镉逆境胁迫存在着一定的应答关系。研究结果也为甜菜耐重金属镉机制研究提供参考依据。

BvGSTU9 Gene in Sugar Beet: Bioinformatics and Expression Analysis Under Cadmium Stress

To further study the function of BvGSTU9 (LOC104894060) during the heavy metal stress in sugar beet (Beta vulgaris L.), ‘780016B/12You’ was chosen as the plant material in this study to forecast and analyze the sequence characteristics, structure and function of the gene. In addition, quantitative real time polymerase chain reaction (qRT-PCR) was used to detect the changes of expression under different concentrations of cadmium stress. The results show that the whole length of BvGSTU9 gene is 925 bp, and the open reading frame is 675 bp, encoding an unstable extracellular protein composed of 224 amino acids. The amino acid sequence of BvGSTU9 is similar to that of spinach and quinoa, and it is almost consistent with the phylogenetic evolution tree analysis. The prediction of the secondary structure and the tertiary structure indicate that the gene is mainly composed of α-Spiral, β-Folding, extending chain and irregular curling. qRT-PCR analysis show that BvGSTU9 gene is induced in different degrees by different concentrations of cadmium stress. Therefore, it can be inferred that the BvGSTU9 gene in sugar beet has a certain response relationship with cadmium stress both in structure and function. The results also provide a certain reference basis for the study on the mechanism of heavy metal cadmium tolerance of sugar beet.