毛栓菌27-1漆酶对酚酸类化合物降解的效能分析

探究毛栓菌27-1漆酶对酚酸类化合物的降解效能,为竹林地酚酸类自毒物质降解提供依据。采用超高效液相色谱检测法,通过测定菌液处理后酚酸含量变化,研究毛栓菌27-1漆酶对10种酚酸类化合物的降解特性。结果表明,毛栓菌27-1漆酶能完全降解酚酸类化合物中的香草酸、咖啡酸和芥子酸;丁香酸、阿魏酸和龙胆酸的降解效果一般,降解率50%左右;原儿茶酸的降解效果较差,降解率达35%;不能降解香豆酸、对羟基苯甲酸和绿原酸。研究不同条件下毛栓菌27-1漆酶降解酚酸类化合物丁香酸的效能,发现在pH 5、温度30℃、丁香酸初始浓度小于100 mg/L时,菌株对丁香酸具有高效降解能力,ABTS溶液作为介体物质加入降解体系后降解效果更好,在浓度为0.5 mmol/L时,降解率可达到100%。毛栓菌27-1产生的漆酶对酚酸类化合物具有良好降解效能。

The Degradation Efficiency of Trametes hirsuta 27-1 Laccase on Phenolic Acids

The study aims to investigate the degradation efficiency of phenolic acid compounds by Trametes hirsuta 27-1 laccase so as to provide a basis for the degradation of phenolic acids in bamboo forest. Ultra-high performance liquid chromatography was used. By measuring the change of phenolic acid content after bacterial solution treatment, we explored the degradation properties of 10 kinds of phenolic acid compounds by Trametes hirsuta 27-1 laccase. The results show that laccase could completely degrade vanillic acid, caffeic acid and sinapic acid; the degradation effect of syringic acid, ferulic acid and gentian acid are general and the degradation rate is about 50%; the degradation effect of protocatechuic acid is poor, the degradation rate could be 35%, and coumaric acid, p-hydroxybenzoic acid and chlorogenic acid could not be degraded. By studying the efficacy of Trametes hirsuta 27-1 laccase in the degradation of syringic acid under different conditions, it is found that, under the condition of pH 5, temperature of 30℃ and the initial concentration of syringic acid less than 100 mg/L, the strain could have high degradation ability of syringic acid. After ABTS solution is added to the degradation system as a mediator, the degradation effect is much better. At a concentration of 0.5 mmol/L, the degradation rate could reach 100%. In conclusion, the laccase produced by Trametes hirsuta 27-1 has good degradation efficiency on phenolic acids.