| 基于高通量测序方法的蒙古栎SSR标记开发 |
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| 引用本文： | 陈罡,于世河,姜义仁,卜鹏图,郑颖,冯健.基于高通量测序方法的蒙古栎SSR标记开发[J].中国农学通报,2021,37(16):13-17. |
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| 作者姓名： | 陈罡 于世河 姜义仁 卜鹏图 郑颖 冯健 |
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| 作者单位： | 1.辽宁省林业科学研究院,沈阳 110032;2.沈阳农业大学生物科学技术学院,沈阳 110866 |
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| 基金项目： | “十三五”国家重点研发计划项目子课题“蒙古栎优良种源选择及优质用材产量形成机制研究”(2017YFD0600602-02);“十三五”国家重点研发计划项目子课题“温带日本落叶松高效培育技术研究”(2017YFD060040103);辽宁省农业科学研究院学科建设计划任务“林木遗传育种学科建设”(2019DD217032);辽宁省“兴辽英才计划”项目(XLYC1902081) |
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| 摘 要： | 为研究蒙古栎遗传多样性,基于Illumina高通量de novo拼接序列数据鉴定10个SSR位点,并利用8个采自辽宁不同地区的蒙古栎样本进行多样性验证。结果表明,这10个新SSR位点具有一定多态性,每个位点的等位基因数为4~6,平均为4.5个,这批针对蒙古栎专门开发的SSR标记对于研究辽宁分布的蒙古栎群体遗传多样性和资源利用将具有很大价值。
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| 关 键 词： | 蒙古栎 SSR 高通量测序 引物开发 遗传多样性 |
| 收稿时间： | 2020-07-30 |
Development of SSR Markers for Quercus mongolica Based on High-throughput Sequencing |
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| Chen Gang,Yu Shihe,Jiang Yiren,Bu Pengtu,Zheng Ying,Feng Jian.Development of SSR Markers for Quercus mongolica Based on High-throughput Sequencing[J].Chinese Agricultural Science Bulletin,2021,37(16):13-17. |
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| Authors: | Chen Gang Yu Shihe Jiang Yiren Bu Pengtu Zheng Ying Feng Jian |
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| Institution: | 1.Liaoning Academy of Forestry Sciences, Shenyang 110032;2.College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866 |
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| Abstract: | In order to study the genetic diversity of Quercus mongolica, 10 SSR loci were identified based on Illumina high-throughput de novo splice sequence data, and the diversity of 8 Quercus mongolica samples from different areas in Liaoning Province were verified. The result showed that the number of alleles per locus varied from 4 to 6, with a mean of 4.5. These new SSR markers would be useful for further investigation of genetic diversity and germplasm utilization of Quercus species. |
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| Keywords: | Quercus mongolica SSR high-throughput sequencing primer development genetic diversity |
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