| 鸡传染性支气管炎病毒地方分离株S1、N和M基因的克隆与表达 |
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| 引用本文： | 闫芳,赵宇军,吴倩,岳文斌,李绪英,吉文汇,刘娟,刘风波,任家琰,化丽珍.鸡传染性支气管炎病毒地方分离株S1、N和M基因的克隆与表达[J].中国农学通报,2010,26(16):14-17. |
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| 作者姓名： | 闫芳 赵宇军 吴倩 岳文斌 李绪英 吉文汇 刘娟 刘风波 任家琰 化丽珍 |
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| 作者单位： | 山西农业大学动物科技学院,山西,太谷,030801 |
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| 基金项目： | 山西省自然科学基金，山西高校科技研究开发项，山西农业大学博士后基金 |
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| 摘 要： | 摘要：将IBVSX16株的S1、N和M基因克隆入真核表达载体pVAX1，构建IBV真核表达载体pVAX1-16S1、pVAX1-16N和pVAX1-16M。体外转染BHK-21细胞，用间接ELISA检测到目的蛋白表达，用构建的3个表达载体免疫小鼠，从小鼠血清中检测到了抗IBV抗体，初步证实用IBVS1、N和M基因作为核酸疫苗免疫动物，可以激活机体免疫应答。
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| 关 键 词： | 陆海回交群体 陆海回交群体 遗传图谱 产量性状 QTL |
| 收稿时间： | 2010/3/24 0:00:00 |
| 修稿时间： | 2010/4/26 0:00:00 |
Cloning and Expression of the S1, N and M gene of IBV |
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| Yan Fang,Zhao Yujun,Wu Qian,Yue Wenbin,Li Xuying,Ji Wenhui,Liu Juan,Liu Fengbo,Ren Jiayan,Hua Lizhen.Cloning and Expression of the S1, N and M gene of IBV[J].Chinese Agricultural Science Bulletin,2010,26(16):14-17. |
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| Authors: | Yan Fang Zhao Yujun Wu Qian Yue Wenbin Li Xuying Ji Wenhui Liu Juan Liu Fengbo Ren Jiayan Hua Lizhen |
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| Institution: | (College of Animal Science and Technology shanxi Agricultural University,Taigu Shaanxi 030801) |
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| Abstract: | For efficacious DNA vaccine development against infectious bronchitis virus（IBV）,we constructed plasmids containing S1,N and M protein of virulent IBV.These plasmids were tested by transient transfection assays in BHK-21 cells and in mice.The S1,N and M genes of IBVSX16 strain were sub-cloned into the eukaryotic expression plasmid pVAX1,which is driven by CMV promoter,named pVAX1-16S1,pVAX1-16N and pVAX1-16M,respectively.Purified plasmids DNA were transfected into BHK-21 cells by lipofectamine.The IBV S1,N and M proteins were expressed in BHK-21,which do specifically react to the CDV antisera.The mice inoculated with these plasmids induced the immune response against IBV. |
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| Keywords: | IBV S1 gene N gene M genes |
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