嗪草酮高效降解菌N1发酵培养基优化研究

旨在研究嗪草酮高效降解菌N1菌株的培养基最优组成配方，为降解菌提供最优发酵条件，从而为降解菌扩大生产提供理论依据。应用正交试验设计对培养基组分进行优化。结果表明，单因素试验中最优碳源和氮源分别为蔗糖和酵母粉，最优添加量为0.3%和0.1%。对降解菌生长有促进作用的无机盐有K₂HPO₄、NaCl、KH₂PO₄、MgSO₄·7H₂O。嗪草酮高效降解菌N1的最佳发酵培养基组成为：pH 7.0，K₂HPO₄ 0.15%，蔗糖0.3%，酵母粉0.15%，NaCl 0.1%，KH₂PO₄ 0.05%，MgSO₄·7H₂O 0.04%。

Study on Fermentation Medium Optimization of Metribuzin Degrading Bacteria N1

The paper aimed to study the medium optimal composition formula of metribuzin efficient degradation bacteria strain N1,and provided optimal fermentation conditions for degradation bacteria strain N1,thus provided a theoretical basis for the expand production of degradation bacteria strain N1.Used the orthogonal experimental design optimize the medium components.Optimal carbon and nitrogen sources in the single-factor test respectively were sucrose and yeast,the optimal dosage were 0.3% and 0.1%.The inorganic salts which can promote the growth of degradation bacteria were K2 HPO4,NaCl,KH2 PO4,MgSO4 7H2 O.The optimum fermentation medium composition of metribuzin efficient degradation bacteria N1 were as follows：pH 7.0,K2 HPO4 0.15%,sucrose 0.3%,yeast 0.15%,NaCl 0.1%,KH2 PO4 0.05%,MgSO4·7H2 O 0.04%.