家蚕交配囊精液的活化与人工授精

采用姬姆萨染色及显微镜下直接观察家蚕交配囊中精液，并用交配囊精液进行人工授精，研究发现：交配20~30min雌蛾交配囊中精液与雄蛾储精囊中精液一样，精液未被活化，含有大量有核精子束。用不同浓度的胰蛋白酶处理精液后在显微镜下观察与人工授精的结果显示，0.2μg/ml的胰蛋白酶处理精液受精卵得率最高。用胰蛋白酶、血纤维蛋白溶酶、胃蛋白酶及琥珀酸钠处理精液，仅胰蛋白酶能够在很好的溶解有核精子束的同时提高无核精子的运动性，也只有胰蛋白酶处理的精液人工授精能够得到受精卵。研究还发现经2倍稀释的精液人工授精效果最佳。

Activation and artificial insemination using bursa copulatrix sperm of silkworm, Bombyx mori

Uses giemsa's dye and microscope observe bursa copulatrix sperm of silkworm directly, artificial insemination using the sperm, the studies shows: The seminal fluid is similar in the male moth seminal vesicle and in the female bursa copulatrix after mating 20 to 30 minute, and the seminal fluid is contains large number of eupyrene sperm bundles and not be activated. The result of microscope observation and artificial insemination using sperm treated with diffirent trypsin concentration shows that 0.2μg/ml trypsin in 50% semen solution, the fertilization rate is highest. The sperm treated with trypsin, plasmin, pepsin and succinic acid sodium respectively, only trypsin can dissociate the eupyrene sperm bundles and improve the motility of apyrene sperm synchronously , and olso only the trypsin-treated sperm have the ability of fertilization. The optimum concentration of sperm for artificial insemination is 2-fold diluted still be found in the research.