两种细菌学检查方法的比较

摘 要: 【目的】采用两种细菌学检查方法对牛乳中细菌进行菌数测定，比较得出最佳检测方法。【方法】采用显微镜计数法、标准平板计数法进行测定。【结果】微生物显微镜直接计数法随机性大,对菌体数量不能做出较为宏观,全面的反应.优点是计数快速；平板菌落计数法速度慢,需要平板上长出菌落一段时后才能计数。【结论】由于平板菌落计数法通常间做梯度稀释,所以计数的线性范围大.由于是菌悬液涂布,所以比较均匀,能较好的反应菌落的疏密程度.重复性,平行性很好,是经典的计数方法。

Comparison of two methods in the inspection of bacteria

Compare two bacteriological inspection methods in detecting the bacteria in cow’s milk, and to find a better detection method. The microscope count and the standard plating count methods were performed on the determination of the number of bacteria in the fresh cow＇s milk. Microorganism microscope direct count method had big randomness. It can not obtain macroscopic and comprehensive evaluation of the milk quantity, but it was high-efficient. The plating colony count method was relatively slow and needed a period of time for the bacteria to grow on the plate. The plating count method usually performs the gradient dilution and therefore the counting linear scope is broad. Additionally, the bacterium suspended solution can be distributed uniformly on the plate, so the density of the bacterial colony can be well reflected. The plating count method is a classical method of counting bacteria.