SRAP标记技术在花生种子纯度鉴定中的应用

研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性(SRAP)。结果表明, SARP技术可以揭示花生栽培种的遗传差异。在所试验的74对SRAP引物中,2对只获得了单态性条 带,其余72对总共产生了1,812条带,其中1035条(57．12％)为多态性带,每对引物组合平均获得25．16 条带、14．38条多态性带。在这73对引物组合中,总带数和多态性条带的数目分别为7-63和2-44 条。10个花生栽培种的简单匹配相似系数为0．6967-0．9171不等,根据SRAP指纹图谱进行聚类分析 可将这10个品种分为5类。用64对引物筛选作图亲本24-3与B4,获得329条多态性条带。此项研究 为花生品种鉴定和遗传研究提供了新的DNA分子标记技术手段。

Study on the Seed Test Using Sequence-related Amplification Polymorphisms (SRAP) in Cultivated Peanut (Arachis hypogaea L.)

SRAP assay was conducted using 10 peanut cultivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. Among the 74 primer pairs tested, 2 produced mono-morphic bands only, and seventy-two totally produced 1,812 bands, among which 1035 (57.12%) were polymorphic, with an average of 14.38polymorphic bands and 25.16 bands per primer combination. The total number of bands and polymorphic bands in the 74 primer combinations ranged from 7-63 and 2-32 respectively. The simple matching coefficient of the peanut varieties ranged from 0.6967 to 0.9171. The varieties may be grouped into 5 categories based on cluster analysis of the SRAP fingerprinting data. In a separate study on SARP using 64 primer pairs, 329 polymorphic bands were detected in 24-3 and B4,the parental genotypes for mapping. This study provided an additional marker system for peanut variety identification and genetic research.