| 优良木薯品种“ND-50”的组织培养 |
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| 引用本文： | 何婷,孙月芳,王亦菲,陆瑞菊,邹磊,陈志伟,杜志钊,张燕敏,黄剑华.优良木薯品种“ND-50”的组织培养[J].中国农学通报,2010,26(3):52-54. |
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| 作者姓名： | 何婷 孙月芳 王亦菲 陆瑞菊 邹磊 陈志伟 杜志钊 张燕敏 黄剑华 |
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| 作者单位： | 上海市农业科学研究院生物技术研究所,上海,201106;上海市农业遗传育种重点实验室,上海,201106 |
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| 摘 要： | 研究优良品种木薯"ND-50"无性系组织培养技术。以木薯品种"ND-50"带腋芽的茎段为外植体进行组织培养,在芽的增殖和继代生长两个阶段进行观察研究。结果表明,2/3MS+6-BA 1.0 mg/L+IAA1.0 mg/L培养基较适合作为组培苗的增殖培养基;2/3MS+KT 1.0 mg/L+NAA0.05 mg/L作为组培苗的壮苗培养基较好。通过"ND-50"组织培养研究,为木薯的快速繁育和推广提供参考依据。
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| 关 键 词： | 大豆 大豆 连作 根际 土壤养分 |
| 收稿时间： | 2009-09-14 |
| 修稿时间： | 2009-10-23 |
Tissue Culture of Manihot esculenta Crantz ND-50 |
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| He Ting,Sun Yuefang,Wang Yifei,Lu Ruijiu,Zou Lei,Chen Zhiwei,Du Zhizhao,Zhang Yanmin,Huang Jianhua.Tissue Culture of Manihot esculenta Crantz ND-50[J].Chinese Agricultural Science Bulletin,2010,26(3):52-54. |
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| Authors: | He Ting Sun Yuefang Wang Yifei Lu Ruijiu Zou Lei Chen Zhiwei Du Zhizhao Zhang Yanmin Huang Jianhua |
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| Institution: | He Ting~(1,2),Sun Yuefang~(1,Wang Yifei~(1,Lu Ruijiu~(1,Zou Lei~(1,Chen Zhiwei~(1,Du Zhizhao~(1,Zhang Yanmin~(1,Huang Jianhua~(1,2) (1 Biotech Research Institute,SAAS,Shanghai 201106,2 Shanghai Key Laboratory of Agricultural Genetics , Breeding,Shanghai 201106) |
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| Abstract: | The research aimed to study the tissue culture of varieties ND-50 of cassava by using its node sections taken as explants including multiplication and subculture.The results showed that 2/3MS+ 6-BA 1.0 mg/L+IAA 0.1 mg/L was suitable for the multiplication medium of tissue culture seedling.2/3MS+KT 1.0 mg/ L + NAA 0.05 mg/L was better for subculture medium.The tissue culture of cassava ND-50 could provide references for its seedlings rapid propagation and promotion. |
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| Keywords: | ND-50 |
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