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| 甜瓜作图亲本相关序列扩增的多态性分析 | |
| 引用本文： | 姚建春,刘玲,李唯,王建设.甜瓜作图亲本相关序列扩增的多态性分析[J].中国农学通报,2006,22(7):59-63. |
| 作者姓名： | 姚建春 刘玲 李唯 王建设 |
| 作者单位： | 1. 甘肃农业大学,兰州,730070;国家蔬菜工程技术研究中心,北京,100089 2. 国家蔬菜工程技术研究中心,北京,100089 3. 甘肃农业大学,兰州,730070 |
| 基金项目： | 国家高技术研究与发展计划“863”项目（No.2002AA244021）与北京市重大科技合同项目（No.H022020130130）资助. |
| 摘 要： | 以中国甜瓜地方品种自交系4G21(香瓜)和3A832(哈密瓜)为作图亲本,利用SRAP(Se-quence-relatedAmplifiedPolymorphism)分析了甜瓜作图亲本的多态性,结果表明:在184个SRAP引物组合中有176个扩增出多态性条带,不同引物组合扩增的多态性条带的变异幅度在1～15之间,共扩增出614个,平均每个引物组合扩增出3.34个,说明SRAP标记揭示甜瓜作图亲本多态性的效率很高。 |
| 关 键 词： | 甜瓜 作图亲本 SRAP 多态性 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-102006-03-21 |
Study of Sequence-related Amplified Polymorphism of Mapping Parents of Melon (Cucumis melo L.) |
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| Yao Jianchun,Liuling,Liwei,Wang Jianshe.Study of Sequence-related Amplified Polymorphism of Mapping Parents of Melon (Cucumis melo L.)[J].Chinese Agricultural Science Bulletin,2006,22(7):59-63. | |
| Authors: | Yao Jianchun Liuling Liwei Wang Jianshe |
| Institution: | 1.Gansu Agricultural University, Lanzhou 730070; 2.National Engineering Research Center for Vegetables, Beijing 100089 |
| Abstract: | SRAP(Sequence-related Amplified Polymorphism)was applied in mapping parents of melon (Cucumis melo L.) analysis .184 SRAP primer pairs were used to detect the polymorphisms between 4G21 (sp,dudaim) and 3A832(Cucumis melo L.) and showed 176 primer pairs generated 614 polymorphic bands with an average of 3.34 polymorphic bands per primer pair. The primer pairs showing polymorphism produced 1~15 polymorphic bands. The result showed that SRAP marker has high polymorphism to mapping parents of melon. |
| Keywords: | Melon(Cucumis melo L ) Mapping parents SRAP Polymorphism |
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