X射线灭活活体日本沼虾精子的初步研究

为检测X射线对日本沼虾精子的灭活效果,利用X射线对雄性日本沼虾进行不同时间的照射处理,将处理后的雄虾与刚完成生殖脱壳的雌虾交配。待雌虾产卵后,计数雌虾抱卵天数,并在显微镜下观察卵的畸形情况。X射线管照射强度为100 k V,5 m A,源距10 cm,照射时间为0、30、60、90、120 min。结果发现:照射30 min组的抱卵天数及卵的畸形率较0 min组无变化,卵的畸形率为0,卵能正常发育至孵化出膜。随着照射时间的增加,在30~90 min之间,雌虾抱卵天数逐渐减少,且所抱卵的畸形率呈上升趋势。照射120 min组的抱卵天数较90 min组变化不大,卵的畸形率达100%。在本试验照射时间范围(0~120 min)内,灭活日本沼虾精子遗传物质的最佳照射时间为90~120 min。

Effect of X-ray on Inactivating Sperms by Radiating Intravital Macrobrachium nipponensis

To investigate the effect of X-ray on sperm inactivation of Macrobrachium nipponense, male shrimps were irradiated by 100 kV-5 mA X-ray for 0, 30, 60, 90, 120 min with a distance of 10 cm. Then the male shrimps were made to mate with female soft-shelled shrimps. Broody days and aberration rate were recorded after ovulation. The results showed that there was no difference between 0 min group and 30 min group, and the aberration rate was 0. Broody days gradually decreased while aberration rate increased with increasing irradiation time from 30 to 90 min. After 90 min, broody days showed no change, and the aberration rate reached a peak of 100%. In this research, the radiation time range was 0-120 min. According to the results, the optimum irradiation time to inactivate genetic material of sperms of Macrobrachium nipponense was 90- 120 min.