| 山新杨组织培养快繁技术研究 |
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| 引用本文： | 刘文萍,韩玉琴,南相日,朱 财.山新杨组织培养快繁技术研究[J].中国农学通报,2005,21(1):101-101. |
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| 作者姓名： | 刘文萍 韩玉琴 南相日 朱 财 |
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| 作者单位： | (黑龙江省农科院生物技术研究中心， 哈尔滨 150086) |
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| 摘 要： | 山新杨冬季枝条经FC处理，选择萌发新枝的茎段和叶片为接种外植体；诱导茎段产生腋芽的培养基为1/2MS+6-BA0.2~0.5mg/L+NAA0~0.5mg/L，诱导叶片产生不定芽的培养基为1/2MS或MS+6-BA0.5mg/L+NAA0.5mg/L；丛生芽快繁培养基MS+6-BA0.3mg/L+NAA0.3mg/L，每20天继代一次，繁殖倍数20左右；生根培养基为1/2MS或MS，附加0.1~0.5mg/L的多效唑，对生根有促进作用。
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| 关 键 词： | 山新杨 组织培养 快繁技术 |
Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena ) |
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| Liu Wenping,Han Yuqin,Nan Xiangri,Zhu Cai.Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena )[J].Chinese Agricultural Science Bulletin,2005,21(1):101-101. |
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| Authors: | Liu Wenping Han Yuqin Nan Xiangri Zhu Cai |
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| Institution: | (Biotechnological Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086) |
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| Abstract: | Stem segments and leaves of new shooting, which are from winter shoot treated by FC , was used as inoculating explant. The medium used for producing axillary bud from stem segment is 1/2MS+6-BA0.2~0.5mg/L + NAA 0~0.5mg/L; the medium used for generating adventitious bud is 1/2MS or MS + 6-BA 0.5mg/L + NAA0.5mg/L; medium used for producing rosette buds is MS+6-BA0.3mg/L + NAA0.3mg/L. Regeneration was conducted every 20 days and 20 times of multiplication could be achieved. Rooting medium is 1/2MS or MS. Adding benzimidazole 0.1~0.5mg/L in the medium could promote rooting. |
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| Keywords: | Shanxinyang(P davidiana X P bollena )zz Tissue culturezz Rapid multiplication techniqueszz |
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