| 南方根结线虫延长因子2基因cDNA全长克隆和序列分析 |
| |
| 引用本文： | 黎娟华 赵平娟 孙海彦 彭,明.南方根结线虫延长因子2基因cDNA全长克隆和序列分析[J].中国农学通报,2011,27(1):223-228. |
| |
| 作者姓名： | 黎娟华 赵平娟 孙海彦 彭 明 |
| |
| 作者单位： | 中国热带农业科学院热带生物技术研究所,海口,571101 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项项目资助"利用RNAi技术防治南方根结线虫的研究" |
| |
| 摘 要： | 以南方根结线虫为材料，根据同源序列法分别进行3′末端和5′末端的RACE，获得延长因子基因2 cDNA全长。此cDNA全长序列为2434 bp，包括2334 bp的完整ORF，编码一含777个氨基酸的多肽。该基因命名为Mi-eft2，其推导蛋白MI-EFT2的编码氨基酸与秀丽隐杆线虫的Ce-eft1，Ce-eft2，Ce-eft3，Ce-eft4基因的推导蛋白的同源性分别为26.7%、86.9%、13.8%和8.9%。蛋白功能分类预测MI-EFT2是一种在蛋白质延长过程中起作用的GTP水解酶，与真核生物的延长因子2功能相同。
|
| 关 键 词： | 朱蕉 朱蕉 RAPD 形态性状 聚类分析 |
| 收稿时间： | 2010/6/29 0:00:00 |
| 修稿时间： | 9/8/2010 12:00:00 AM |
Cloning and Sequence Analysis of Full-length cDNA of Elongation Factor 2 Gene of Meloidogyn incognita |
| |
| Li Juannhua,Zhao pingjuan,Sun haiyan,Peng Ming.Cloning and Sequence Analysis of Full-length cDNA of Elongation Factor 2 Gene of Meloidogyn incognita[J].Chinese Agricultural Science Bulletin,2011,27(1):223-228. |
| |
| Authors: | Li Juannhua Zhao pingjuan Sun haiyan Peng Ming |
| |
| Institution: | Li Juannhua,Zhao pingjuan,Sun haiyan,Peng Ming (Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101) |
| |
| Abstract: | The full-length cDNA sequence of elongation factor 2 (Mi-eft2) was obtained by RT-PCR and RACE from Meloidogyne incognita. It consisted of 2434 nucleotides, and encoded 777 amino acids. The amino acid sequence of deduced protein MI-EFT2 was 26.7%, 86.9 %, 13.8% and 8.9% identical with the deduced proteins of Ce-eft1, Ce-eft2, Ce-eft3 and Ce-eft4 from Caenorhabditis elegans respectively. Protein function prediction of MI-EFT2 suggested that it was a GTPase just like the eukaryotic elongation factor EF2, invo... |
| |
| Keywords: | RACE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
