我国棉花黄萎病菌基于SSR的遗传多样性分析

为了对棉花黄萎病菌群体的遗传多样性进行研究,以棉花黄萎病菌基因组DNA为模板,用双因素和单因素水平优化的方法对SSR反应体系的重要参数进行摸索和优化,建立了最适反应体系.从300对引物中筛选出13对多态性较高的引物,并对来自我国12个省96个县(市)的170个棉花黄萎病菌株进行SSR分析.SSR图谱的聚类分析结果将供试...

Genetic Diversity of Verticillium dahliae Populations of Cotton in China Based on SSR Analysis

In order to study genetic diversity of Verticillium dahliae populations isolated from cotton, using genome DNA of Verticillium dahliae as template, important parameters of the SSR reaction system were optimized through single-factor and double-factor analysis, and the optimal SSR reaction system for Verticillium dahliae was establishied. 13 pairs of polymorphic higher SSR primers chosen from 300 primers were used to amplify genome DNA of 170 tested isolates of Verticillium dahliae from 96 counties(cities) of 12 provinces in China. In SSR profiles of the cluster analysis, these isolates were classified into two groups: GroupⅠincluded 26 isolates, 18 isolates of this group were from Xinjiang which accouned for 69.23%; GroupⅡincluded 144 isolates, 138 isolates of this group were from the inland which accounted for 95.83%. So there was significant difference between the isolates in Xinjiang and other provinces. SSR fingerprinting exhibited that there was a significant correlation with geographical origin of isolates, no correlation with the pathogenicity, but weak virulence isolates may be more prone to genetic variation.