| 转Bt-CpTI-GNA基因棉花的研究 |
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| 引用本文： | 吴霞,王娇娟,朱祯,上官小霞,张林水,李波,杜春芳,李燕娥.转Bt-CpTI-GNA基因棉花的研究[J].棉花学报,2005,17(6):353-359. |
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| 作者姓名： | 吴霞 王娇娟 朱祯 上官小霞 张林水 李波 杜春芳 李燕娥 |
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| 作者单位： | 1. 山西省农业科学院棉花研究所,山西,运城,044000
2. 中国科学院遗传与发育生物学研究所,北京,100101 |
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| 基金项目： | 国家“863”项目(2004AA212102)，山西省科技攻关项目(021010)，山西省自然科学基金项目(20011091) |
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| 摘 要： | 采用农杆菌介导法将外源三价抗虫基因(Bt-CpTI-GNA)导入常规棉花品种中,获得转基因再生株,分子检测表明外源基因已在棉花体内表达,并遗传给后代材料。PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。其所携带的基因在转基因棉花中有分离现象,这可能是外源基因整合到受体棉株体内“基因沉默”而引起所致。
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| 关 键 词： | 棉花 农杆菌介导转化 Bt-CpTI-GNA 抗虫性 标记基因 |
| 文章编号： | 1002-7807(2005)06-0353-07 |
| 修稿时间： | 2005年3月15日 |
Study of Transgenic Cotton Carrying Bt-CpTI-GNA Genes |
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| WU Xia,WANG Jiao-juan,ZHU Zhen,SHANGGUAN Xiao-xia,ZHANG Lin-shui,LI Bo,DU Chun-fang,LI Yan-e.Study of Transgenic Cotton Carrying Bt-CpTI-GNA Genes[J].Cotton Science,2005,17(6):353-359. |
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| Authors: | WU Xia WANG Jiao-juan ZHU Zhen SHANGGUAN Xiao-xia ZHANG Lin-shui LI Bo DU Chun-fang LI Yan-e |
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| Institution: | WU Xia~1,WANG Jiao-juan~1,ZHU Zhen~2,SHANGGUAN Xiao-xia~1,ZHANG Lin-shui~1,LI Bo~1,DU Chun-fang~1,LI Yan-e~ |
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| Abstract: | Foreign genes(Bt-CpTI-GNA) resistant to cotton insects were introduced into conventional cotton cultivars by Agrobacterium tumefaciens-mediated transformation.Regenerated plants have been obtained.The results of molecular detection indicated that the foreign genes had been transferred into cotton plants.The results of PCR molecular detection,marker genes and foreign target-gene with insect-resistance were quite regular.Some of their progenies' segregation coincided with Mendel's regularity of segregation,but some deviated.This phenomenon may be due to "gene silence". |
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| Keywords: | cotton Agorbacterium tumefaciens-mediated transformation Bt-CpTI-GNA insect-resistance marker gene |
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