海岛棉两种转Bt基因方法的研究

 以新疆海岛棉军海1号为受体，利用农杆菌菌液喷雾法（Floral spray）和质粒注射法（Plasmid injection）导入外源Bt基因。经过对转化植株T₁和T₂代大田筛选和PCR、Southern检测，并经孟德尔遗传规律符合度分析，结果表示：农杆菌菌液喷雾法T₀代成铃率比质粒注射法高8.3百分点；两种方法均可获得转基因植株，农杆菌菌液喷雾法较质粒注射法转化率高3.4百分点；农杆菌菌液喷雾处理后代较符合孟德尔遗传规律且单拷贝插入几率高。因此，农杆菌菌液喷雾法优于质粒注射法。

Comparison of Two Approaches for Transformation Bt in Sea-island Cotton(Gossypium barbadense L.)

By using two different methods, the floral spray and the plasmid injection, the Bt gene was introduced into cotton Junhai 1(Xinjiang sea island cotton) . T1 and T2 transformed progenies were confirmed by field screening, PCR amplification, Southern bolt hybridization and conformity analysis of Mendelian inheritance. The results showed: The boll rate of T0-generation transformed by the floral spray is 8.3 percent approximately higher than that of the plasmid injection. In this study, it has been proved that transgenic plants can be obtained through each of the two methods, and the transformation efficiency of the former is 3.4% higher than that of the latter; the floral spray is more in line with Mendelian inheritance, and probability of single copy is higher. So, the better one is the floral spray.