转Bt基因抗虫棉中棉所30的碳、氮代谢特征

本试验在田间条件下研究了转Bt基因抗虫棉中棉所30的碳、氮代谢特征,于各主要生育期测定了棉株地上部营养器官的全N、非蛋白质N和可溶性糖及淀粉含量.研究结果表明,与轮回亲本中棉所16相比,中棉所30苗蕾期的C/N较低,非蛋白质N含量较高,N代谢比较旺盛;初花期至盛花期的C/N则较高,C代谢明显加强;进入结铃早期,中棉所30叶片中可溶性糖、淀粉及营养体中非蛋白质N的含量均下降,反映出其"根源”和"叶源”的功能已减弱,表现出早衰迹象.为了改善转Bt基因抗虫棉中棉所30的生育状况,提高其产量和品质,"促源”是根本.

Nitrogen and Carbon Metabolizing of Bt Transgenic Cotton CCRI 30 TIAN Xiao-li, YANG Pei-zhu, WANG Bao-

Nitrogen and carbon metabolizing of Bt transgenic cotton CCRI 30 was studied under field condition by measuring the content of total nitrogen,of which is not a component part of protein (PNN), soluble sugar and starch in the vegetative part of cotton plant. The results were as following: (1) In seedling and squaring stage, C/N of CCRI 30 was lower, and the content of PNN was higher compared to its recurrent parent CCRI 16. The nitrogen metabolizing of CCRI 30 was more vigorous in the earlier development stage. (2) In first bloom and full bloom stage, C/N of CCRI 30 was higher than that of CCRI 16. The carbon metabolizing of CCRI 30 had been enhanced significantly. (3) In earlier boll forming stage, the content of soluble sugar and starch in leaves and the content of PNN in vegetative part of the plant of CCRI 30 were all reduced, which suggested that the function of leaf and root have weakened in this stage, showing the sign of early ageing. From the above results, it was obvious that promoting the development of resource organ was fundamental to improve the yield and quality of Bt transgenic cotton CCRI 30.