棉花RGAP、DGAP和DDRT标记的定位研究

 根据本实验室已克隆的RGAs和DGAs，设计48条RGAs和4条DGAs特异引物，以“海7124×TM-1”组合的BC₁群体为材料把5个RGAP和2个DGAP标记定位到棉花的染色体上；以一个高抗黄萎病陆地棉品系5026为材料，在接种黄萎病菌后不同时期提取根部RNA，用mRNA差异显示（DDRT-PCR）技术，获得164个差异片段，根据这些片段设计34对DDRT引物。用已构建好的“海7124×军棉1号”组合的F₂群体，将3个RGAP和5个DDRT定位到了相应的染色体上。用Joinmap3.0软件把两张连锁图谱整合为一张图谱，并将这些标记与抗黄萎病QTLs进行连锁分析。

Mapping of RGAP,DGAP and DDRT Markers in Cotton

According to the sequences of RGAs and DGAs cloned in our laboratory, 48 primers of RGAP and four primers of DGAP were designed, five RGAP and two DGAP markers were located on the chromosomes of cotton genome using a BC₁ population derived from the cross of ‘Hai 7124×TM-1’; the root RNA was extracted in different period after inoculating the isolate of Verticillium dahliae to 5026, a G. hirsutum line high resistant to Verticillium wilt. Totally 164 differential fragments were gotten using mRNA differential display method, and 34 DDRT primers were designed. Three RGAP and five DDRT loci were mapped to the chromosomes using an F₂ population derived from the cross of ‘Hai 7124×Junmian 1’. The two linkage maps were integrated into one  using Joinmap 3.0 software, and the linkage relationship was analyzed between the markers and the QTLs resistant to Verticillium wilt.