新陆早32号、33号的体细胞胚胎发生和植株再生比较研究

以新疆主栽品种新陆早32号、33号和对照YZ1为研究材料,通过不同浓度的激素组合成功诱导获得了体细胞胚,并进一步发育成苗.研究发现,所用的12种激素组合均能有效诱导愈伤组织.虽然IBA+KT组合有利于诱导YZ1和新陆早33号的快速分化,但在增殖生长过程中体细胞胚容易褐化、死亡:在2,4-D+KT组合中,0.1 mg·L...

Comparative Study of Somatic Embryogenesis and Plant Regeneration in Cotton Cultivars from Xinluzao 32 and Xinluzao 33(Gossypium hirsutum L.)

An efficient somatic embryo procedure was developed to regenerate plantlets from hypocotyls of cotton cultivars Xinluzao 32 and Xinluzao 33. Calli were effectively produced on the medium with IBA/KT hormone regime, but calli were browned and went to death gradually during the process of proliferation. Embryonic calli of Xinluzao 33 appeared earlier on media with 0.1 mg·L^-12,4-D+0.1 mg·L^-1 KT hormone combination than on other media. Embryogenic calli were effectively initiated with high concentration of KT and 2,4-D in medium(0.1 mg·L^-1 2,4-D+0.2 mg·L^-1 KT or 0.2 mg·L^-1 2,4-D+0.5 mg·L^-1 KT). The speed of differentiation and embryogenesis of Xinluzao 33 were faster than that of Xinluzao 32. Embryogenic calli of YZ₁ were quickly initiated on media with low concentration(even no) hormone combination; with high concentration hormone of KT, the calli of YZ1 turned brown and went to death easily．The successful regeneration protocol established in this study can be used to improve cotton cultivars by genetic engineering．