| 转基因抗虫棉中cry1Aa基因表达盒的序列测定和检测 |
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| 引用本文： | 孙爻,谢家建,范荫荫,李蕊,彭于发.转基因抗虫棉中cry1Aa基因表达盒的序列测定和检测[J].棉花学报,2011,23(3):224-227. |
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| 作者姓名： | 孙爻 谢家建 范荫荫 李蕊 彭于发 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业部转基因植物环境安全监督检验测试中心,北京,北京,100193
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业部转基因植物环境安全监督检验测试中心,北京,北京,100193;河南农业大学,郑州,410002 |
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| 基金项目： | 国家重大基础研究发展规划项目，转基因新品种培育重大科技项目 |
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| 摘 要： | 从转基因抗虫棉南农6号F1中克隆了一种新的Bt基因表达盒-cry1Aa基因表达盒,与已经测定的我国转基因棉花中的cry1Ac基因和cry1Ab/Ac基因的表达盒序列比较发现,它们在Bt基因与7S UTR的连接区域存在较大差异.根据这个差异区域的序列,设计特异性引物对cry1Aa-F/cry1Aa-R,建立了cry1Aa...
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| 关 键 词： | 转基因棉花 cry1Aa基因 表达盒 PCR检测 |
| 收稿时间： | 2011-01-01; |
The Sequence and Detection of the cry1Aa Gene Expression Cassette in Transgenic Bt Cotton |
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| SUN Yao,XIE Jia-jian,FAN Yin-yin,LI Rui,PENG Yu-fa.The Sequence and Detection of the cry1Aa Gene Expression Cassette in Transgenic Bt Cotton[J].Cotton Science,2011,23(3):224-227. |
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| Authors: | SUN Yao XIE Jia-jian FAN Yin-yin LI Rui PENG Yu-fa |
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| Institution: | SUN Yao1,XIE Jia-jian1,FAN Yin-yin1,LI Rui1,2,PENG Yu-fa1(1.Institute of Plant Protection,Chinese Academy of Agricultural Sciences/State Key Laboratory for Biology of Plant Diseases and Insect Pests/ Inspection Test Center for Environmental Safety of Transgenic Crops(Beijing),MOA,Beijing 100193,China,2.Henan Agricultural University,Zhengzhou,Henan 410002,China) |
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| Abstract: | A new Bt gene expression cassette cry1Aa gene expression cassette was identified from transgenic insect-resistance cotton Nannong 6 F1 in this study.The difference was found located at the connection region between Bt gene and 7S UTR terminator while being compared with the cry1Ac gene and cry1Ab/Ac gene expression cassettes reported previous.Primer pair cry1Aa-F/cry1Aa-R was designed and construct-specific detection method of cry1Ab/Aa gene expression cassette was established based on the difference.Eight ... |
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| Keywords: | transgenic cotton cry1Aa gene expression cassette PCR detection |
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