| 棉花FAD 2-1基因的克隆及其ihpRNA和amiRNA干扰载体的构建 |
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| 引用本文： | 赵立群,李仁,李蔚,谭小力,杨佑明,华金平,郭仰东.棉花FAD 2-1基因的克隆及其ihpRNA和amiRNA干扰载体的构建[J].棉花学报,2011,23(2):189. |
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| 作者姓名： | 赵立群 李仁 李蔚 谭小力 杨佑明 华金平 郭仰东 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100193
2. 江苏大学生命科学研究院,江苏镇江,212013 |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项(2009ZX08005-024B); 中央高校基本科研业务费专项资金(2009-2-06) |
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| 摘 要： | 采用RT-PCR方法克隆了棉花△-12脂肪酸去饱和酶基因(GhFAD2-1)的cDNA全长序列，该基因含有1158 bp的完整开放阅读框。棉花FAD2-1是种子中编码催化油酸形成不饱和脂肪酸的关键酶的基因，选取GhFAD2-1基因中的一段特异的515 bp片段，构建了种子特异性启动子NAPIN调控的ihpRNA干扰表达载体pFGC1008-NAPIN-FAD2-1，同时构建了针对GhFAD2-1基因的人工miRNA表达载体pCAMBIA1302- amiRNA-FAD2-1。
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| 关 键 词： | 棉花△-12脂肪酸去饱和酶基因 ihpRNA amiRNA 高油酸 |
Cloning of Delta-12 Oleate Desaturase Gene FAD2-1 and Construction of Its ihpRNA and amiRNA Interference Vectors from Gossypium hirsutum |
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| ZHAO Li-qun,LI Ren,LI Wei,TAN Xiao-li,YANG You-ming,HUA Jin-Ping,GUO Yang-dong.Cloning of Delta-12 Oleate Desaturase Gene FAD2-1 and Construction of Its ihpRNA and amiRNA Interference Vectors from Gossypium hirsutum[J].Cotton Science,2011,23(2):189. |
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| Authors: | ZHAO Li-qun LI Ren LI Wei TAN Xiao-li YANG You-ming HUA Jin-Ping GUO Yang-dong |
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| Institution: | ZHAO Li-qun1,LI Ren1,LI Wei1,TAN Xiao-li2,YANG You-ming1,HUA Jin-Ping1*,GUO Yang-dong1*(1.College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China,2.Institute of Life Sciences,Jiangsu University,Zhenjiang,Jiangsu 212013,China) |
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| Abstract: | Delta-12 oleate desaturase gene(FAD2-1),which converts oleic acid into linoleic acid,is the key enzyme determining the fatty acid composition of cottonseed oil.In this paper,the full-length cDNA of GhFAD2-1 was cloned using RT-PCR,which contains an open reading frame of 1158 bp.Based on the sequence of GhFAD2-1,pFGC1008-NAPIN-FAD2-1,an ihpRNA gene-silencing vector controlled by a seed-specific promoter was constructed.Also,an amiRNA gene-silencing vector pCAMBIA1302-amiRNA-FAD2-1 was constructed. |
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| Keywords: | Delta-12 oleate desaturase gene GhFAD2-1 ihpRNA interference amiRNA interference high oleic acid contents |
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