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| 棉花枯萎病菌AFLP分子标记体系的建立及初步应用 | |
| 引用本文： | 王晓光,郭庆港,么奕清,李社增,鹿秀云,李宝庆,马平.棉花枯萎病菌AFLP分子标记体系的建立及初步应用[J].棉花学报,2011,23(2):147-153. |
| 作者姓名： | 王晓光 郭庆港 么奕清 李社增 鹿秀云 李宝庆 马平 |
| 作者单位： | 1. 河北农业大学植物保护学院,河北 保定 071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 2. 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 3. 河北省邯郸市农业局,河北邯郸,056001 |
| 基金项目： | 现代农业产业技术体系专项资金 |
| 摘 要： | 建立并优化了基于HaeⅢ/PstⅠ酶切的棉花枯萎病菌AFLP分析的最佳反应体系,并从32对引物组合中筛选出适合该反应体系的引物组合3对.应用该技术对存在于我国的棉花枯萎病菌3号、7号和8号生理小种的标准菌株进行分析,结果表明该技术能够有效地将这3个标准菌株区分开;通过该体系对来自我国4个省的20个棉花枯萎病菌菌株进行分... |
| 关 键 词： | 棉花枯萎病菌 AFLP 分子标记 优化 遗传多样性 |
Establishment and Application of AFLP Analysis System for Fusarium oxysporum f.sp.vasinfectum |
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| WANG Xiao-guang,GUO Qing-gang,YAO Yi-qing,LI She-zeng,LU Xiu-yun,LI Bao-qing,MA Ping.Establishment and Application of AFLP Analysis System for Fusarium oxysporum f.sp.vasinfectum[J].Cotton Science,2011,23(2):147-153. | |
| Authors: | WANG Xiao-guang GUO Qing-gang YAO Yi-qing LI She-zeng LU Xiu-yun LI Bao-qing MA Ping |
| Institution: | WANG Xiao-guang1,2,GUO Qing-gang2,YAO Yi-qing3,LI She-zeng2,LU Xiu-yun2,LI Bao-qing2,MA Ping2*(1.College of Plant Protection,Agricultural University of Hebei,Baoding,Hebei 071000,China,2.Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,IPM Centre of Hebei Province,3.Handan Municipal Agriculture Bureau,Handan,Hebei 056001,China) |
| Abstract: | An amplified fragment length polymorphism(AFLP) analysis system was constructed by using HaeIII/PstI digested DNA to analyze different isolates of Fusarium oxysporum f.sp.vasinfectum.Three primer combinations were selected from 32 primer pairs and employed in the optimized AFLP system.Three reference strains representing physiological races 3,7 and 8 of F.oxysporum f.sp.vasinfectum from China were characterized by the optimized AFLP system.Cluster analysis using data generated by AFLP makers showed the syst... |
| Keywords: | Fusarium oxysporum f sp vasinfectum AFLP molecular marker optimization genetic diversity |
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