大豆G位点近等基因系叶片类囊体蛋白质组比较分析

类囊体是光合作用和电子传递关键载体,是叶绿体核心组分,在植物亚细胞器蛋白质组研究中尤为重要。为了探究大豆叶片类囊体的蛋白质组,以1对遗传背景相似度为97.6%的大豆种皮颜色G位点近等基因系（NIL-G和NIL-Y）为材料,利用SDS-PAGE与分级质谱相结合的方法对大豆叶片类囊体蛋白进行分析。结果表明,在NIL-G和NIL-Y中鉴定到的总蛋白分别为2 170种和1 730种,特异蛋白分别为1 140种和700种,总蛋白和特异蛋白数量在NIL-G中均高于NIL-Y。特异蛋白的GO注释及KEGG通路分析表明,尽管这对近等基因系遗传背景相似度很高,但其叶片类囊体蛋白在生物途径、细胞组分和分子功能方面均存在差异。

Proteomic Comparation Analysis of Thylakoid in Leaves of G-Locus Near Isogenic Line in Soybean

Thylakoid is the key carrier of photosynthesis and electron transport and also the core component of chloroplast, which is particularly important in the study of plant subcellular organelle proteome. In order to explore the proteome of thylakoid in soybean leaves, a pair of G-locus near-isogenic line (NIL-G and NIL-Y) with 97.6% similarity in the genetic background was used as materials. SDS-PAGE and mass spectrometry were used to isolate the thylakoid protein from soybean leaves. The results showed that the number of total protein and specific protein in NIL-G was higher than that in NIL-Y, respectively. The numbers of total protein and specific protein in NIL-G were 2 170 and 1 140, while 1 730 and 700 in NIL-Y. GO annotation and KEGG pathway analysis of these specific proteins showed that although the high similarity among genetic background, there are differences in the biological pathway, cell components, and molecular functions of thylakoid proteins in soybean. This provide a new clue to the future research on thylakoid proteins in soybean.