| 龙葵杂10号向日葵染色体核型分析 |
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| 引用本文： | 丁海燕,汪春林,武燕,任国领,翟祖欢,马军.龙葵杂10号向日葵染色体核型分析[J].作物杂志,2019,35(2):99-220. |
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| 作者姓名： | 丁海燕 汪春林 武燕 任国领 翟祖欢 马军 |
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| 作者单位： | 1 大庆师范学院生物工程学院,163712,黑龙江大庆2 黑龙江省农业科学院经济作物研究所,150080,黑龙江哈尔滨 |
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| 基金项目： | 大庆师范学院博士启动基金(17ZR03);黑龙江省农业科学院引进博士科研启动基金(201507-42);国家特色油料产业技术体系项目(CARS14-1-06) |
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| 摘 要： | 为了探索向日葵染色体核型和染色体制片技术,以向日葵龙葵杂10号为试验材料,探索了低温预处理向日葵根尖细胞对制片效果的影响;使用Photoshop和Excel软件辅助分析染色体图像进行核型分析。结果表明：低温预处理向日葵根尖细胞,可以获得较理想的染色体制片用于核型分析,龙葵杂10号染色体数量为2n=34,核型公式为2n=34=30m+2sm+2M。
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| 关 键 词： | 向日葵 染色体 核型分析 龙葵杂10号 |
| 收稿时间： | 2018-10-26 |
Karyotype Analysis of Chromosome of Sunflower Longkuiza10 |
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| Haiyan Ding,Chunlin Wang,Yan Wu,Guoling Ren,Zuhuan Zhai,Jun Ma.Karyotype Analysis of Chromosome of Sunflower Longkuiza10[J].Crops,2019,35(2):99-220. |
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| Authors: | Haiyan Ding Chunlin Wang Yan Wu Guoling Ren Zuhuan Zhai Jun Ma |
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| Institution: | 1 School of Biological Engineering, Daqing Normal University, Daqing 163712, Heilongjiang, China2 Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150080, Heilongjiang, China |
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| Abstract: | In order to analyze the chromosome karyotype and chromosome production techniques of sunflower Longkuiza10, the effects of low temperature pretreatment of sunflower root tip cells production was studied by using sunflower Longkuiza10 as the experimental material. Photoshop and Excel software were used to assist in the analysis of chromosome images. The results showed that the low temperature pretreatment of sunflower root tip cells could obtain more ideal chromosome production for karyotype analysis, and the chromosome number of sunflower Longkuiza10 was 2n=34, and the karyotype formula was 2n=34=30m+2sm+2M. |
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| Keywords: | Sunflower Chromosome Karyotype analysis Longkuiza10 |
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