waxy基因功能标记开发及在糯玉米育种中的应用

为利用分子标记辅助选择加快糯玉米种质资源改良进程,以糯玉米自交系云539和普通玉米自交系昌7-2为材料,通过DNA序列测定和分析,探明糯玉米自交系云539 wx基因属于wx-D7突变类型。根据2个自交系基因组序列差异,开发出waxy基因功能标记FMD7,并通过了表型验证和通用性验证。利用开发的基因功能标记FMD7进行分子标记辅助选择,结合产量、株型和品质性状分析,获得携带wx基因的优良昌7-2改良系6个。利用本研究开发的基因功能标记FMD7进行辅助选择可加快回交转育进程,提高育种效率,为选育高产糯玉米杂交种提供材料基础,同时也为分子标记辅助选择单基因或少数主效基因控制的性状提供理论参考。

The Development of waxy Gene Function Marker and Its Application in Waxy Maize Breeding

To accelerate the improvement of waxy maize germplasm resources by molecular marker-assisted selection, waxy maize inbred line Yun 539 and ordinary maize inbred line Chang 7-2 were used. Through DNA sequence analysis, it was found that the wx gene mutation of Yun 539 belongs to wx-D7 mutation. The waxy gene function marker FMD7 was developed based on sequence difference between the two inbred lines and it was identified through phenotypic verification and universal verification. By using the marker FMD7 for molecular marker-assisted selection combined with yield, plant type, and quality trait analysis, six excellent Chang 7-2 improved lines carrying wx gene were identified. The marker FMD7 developed in this study for molecular selection can speed up the process of backcross breeding, improve the breeding efficiency, and provide a material basis for breeding high-yielding waxy maize hybrids. At the same time, it provides a theoretical reference for molecular marker-assisted selection of traits controlled by single gene or a few major genes.