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玉米自交系萌发期耐盐性鉴定
引用本文:段雅娟,曹士亮,于滔,李文跃,杨耿斌,王成波,刘宝民,刘长华.玉米自交系萌发期耐盐性鉴定[J].作物杂志,2022,38(1):213-163.
作者姓名:段雅娟  曹士亮  于滔  李文跃  杨耿斌  王成波  刘宝民  刘长华
作者单位:1黑龙江大学现代农业与生态环境学院,150080,黑龙江哈尔滨2黑龙江省农业科学院玉米研究所,150086,黑龙江哈尔滨
基金项目:黑龙江省农业科学院杰出青年基金“基于测序分型SNP的玉米种质基础分析与杂交组合预测研究”(2019JCQN 004);国家博士后项目“玉米萌发期耐冷性相关分子标记发掘与筛选验证”(2017M621318);黑龙江省农业科学院农业科技创新跨越工程“玉米全株综合加工利用研究”(HNK2019CX22-13)。
摘    要:为了完善玉米自交系萌发期耐盐性的鉴定评价标准,筛选耐盐性强的玉米种质资源,解析玉米自交系萌发期耐盐性遗传机制,为玉米萌发期耐盐性育种提供理论依据和技术支撑。对390份玉米自交系耐盐性相关的10个生理指标进行测定,利用主成分分析确定为5个综合指标,即7d发芽率、芽干重、芽鲜重、根干重和根长,可作为玉米萌发期耐盐性鉴定指标。根据隶属函数分析确定各材料的耐盐综合评价指数,并通过聚类分析将材料萌发期耐盐性分为6个等级,其中高度盐敏感型材料298份,中度盐敏感型材料49份,盐敏感型材料27份,耐盐型材料9份,中度耐盐型材料5份,高度耐盐型材料2份。

关 键 词:玉米  萌发期  耐盐性  主成分分析  聚类分析  
收稿时间:2021-08-18

Identification of Salt Tolerance during Germination of Maize Inbred Lines
Duan Yajuan,Cao Shiliang,Yu Tao,Li Wenyue,Yang Gengbin,Wang Chengbo,Liu Baomin,Liu Changhua.Identification of Salt Tolerance during Germination of Maize Inbred Lines[J].Crops,2022,38(1):213-163.
Authors:Duan Yajuan  Cao Shiliang  Yu Tao  Li Wenyue  Yang Gengbin  Wang Chengbo  Liu Baomin  Liu Changhua
Institution:1College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China2Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
Abstract:In order to optimize the evaluation criteria for salt tolerance of maize inbred lines during germination period, we selected maize varieties with strong salt tolerance, analyzed the genetic mechanism of salt tolerance in maize inbred lines during germination period, which provide important theoretical basis and technical support for salt tolerance breeding in maize germination period. The ten physiological indexes related to salt tolerance of 390 maize inbred lines were measured, and five independent comprehensive indexes (the 7-day germination rate, the 7-day bud dry weight, the 7-day bud fresh weight, the 7-day root dry weight and root length) were determined by principal component analysis. The five comprehensive indexes can be used as the identification indicators of salt tolerance during the germination period of maize. According to the membership function analysis, the comprehensive salt tolerance index of each material was determined, and on this basis, through cluster analysis, the test materials were divided into six grades, according to salt-tolerance during germination period, including 298 high salt-sensitive inbred lines, 49 moderate salt-sensitive inbred lines, 27 salt-sensitive inbred lines, nine salt-tolerant inbred lines, five moderate salt-tolerant inbred lines, and two high salt-tolerant inbred ines.
Keywords:Maize  Germination period  Salt tolerance  Principal component analysis  Cluster analysis
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