| 水稻密穗突变体A989突变基因克隆和转基因植株分析 |
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| 引用本文： | 黎凌,时振英,沈革志,王新其,安林升,张景六.水稻密穗突变体A989突变基因克隆和转基因植株分析[J].作物学报,2010,36(6):887-894. |
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| 作者姓名： | 黎凌 时振英 沈革志 王新其 安林升 张景六 |
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| 作者单位： | 1中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室，上海200032；2上海农业科学院作物研究所，上海201106；3上海交通大学农业与生物学院，上海200240 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从水稻T-DNA插入突变群体中分离得到一个密穗突变体A989,表现晚开花、穗二级枝梗和小花数增加以及包颈。分子检测证明A989中T-DNA是单拷贝插入,通过inverse PCR的方法分离得到A989中T-DNA的旁邻序列,表明T-DNA插入在RCN2基因polyA加A位点后330bp处;RT-PCR检测发现在A989中,RCN2基因的表达被显著上调。利用2×35S启动子在野生型水稻Zhonghua 11中超表达RCN2基因,转基因植株表现为不抽穗,通过细胞学观察发现转基因植株能完成营养生长向生殖生长的转变,但是生殖生长期的分生组织在分化出二级枝梗原基后停止分化和生长。此外,通过比较部分开花相关基因在野生型Zhonghua 11、突变体A989中的表达,推测了RCN2基因可能的作用途径。
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| 关 键 词： | 水稻 T-DNA 密穗 RCN2基因 |
| 收稿时间： | 2010-01-08 |
Dense-Panicle-Related Gene Cloning from Rice Mutant A989 and Transgenic Plant Analysis |
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| LI Ling,SHI Zhen-Ying,SHEN Ge-Zhi,WANG Xin-Qi,AN Lin-Sheng,ZHANG Jing-Liu.Dense-Panicle-Related Gene Cloning from Rice Mutant A989 and Transgenic Plant Analysis[J].Acta Agronomica Sinica,2010,36(6):887-894. |
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| Authors: | LI Ling SHI Zhen-Ying SHEN Ge-Zhi WANG Xin-Qi AN Lin-Sheng ZHANG Jing-Liu |
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| Institution: | 1.National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;2.The Plant Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;3.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China |
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| Abstract: | Rice(Oryza sativa L.) is a model monocotyledonous plant for genetic study due to its small genome size.Along with the completion of genome sequencing,gene cloning and function study becomes the most important task.The concomitant methodology of reverse genetics has played fundamental roles in identifying and studying rice genes in recent years.Rice heading time and inflorescence architecture are two inter-relating and agriculturally important characters.In Arabidopsis,TFL1 gene takes part in the configurati... |
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| Keywords: | T-DNA |
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