反义TRX s基因对转基因小麦籽粒硫氧还蛋白还原活性的影响

以转反义TRX s基因小麦纯合系为材料，分析了转基因小麦TRX h活性和清蛋白、球蛋白、谷蛋白和麦醇溶蛋白各组分及其还原状态的变化。结果表明，不同成熟期转基因小麦籽粒和胚乳中TRX h活性平均比对照降低76.2%和14.2%，其籽粒中的清蛋白、球蛋白、麦醇溶蛋白和谷蛋白巯基含量平均比对照分别低41%、44%、14%和13%，胚乳中分别

Effect of Anti-TRX s Gene on Thioredoxin Reduction Characteristics in Seeds of Transgenic Wheat

Thioredoxins(TRX) are small ubiquitous proteins,containing a readily reducible disulfide bridge.TRX protein plays a role in a wide variety of physiological processes by acting as general protein disulfide reductases and has been proved to have a function of accelerating seed germination.Anti-TRX s gene transferred to wheat can decrease thioredoxin expression and reduce its activity in wheat,so that the pre-harvest sprouting at mature stage can be slowed down.We have obtained the transgenic wheat(Triticum aestivum L.) with antiTRX s gene,and the T_3 and T_4 generations of its pure line 00T89.In the present study,the T_3 and T_4 seeds of 00T89 were planted in both greenhouse and field to investigate TRX h activity,protein components(albumins,gliadins,globulins and glutelins) and free-SH contents in grains and endosperm,with non-transgenic plant as control.The seeds at same panicle position and with same anthesis date were sampled at milk,dough and mature stages,respectively for measurement.The results showed that the TRX h activities in seed and endosperm of transgenic plant were lower than those of control by 76.2% and 14.2% at different mature periods.The free-SH contents of albumins,gliadins,globulins and glutelins were lower by 37%,17%,45% and 6% in seed,and 37%,17%,45% and 6% in endosperm,respectively.However,the contents of albumins,gliadins,globulins and glutelins in seeds increased more than those in control at different mature periods.The results suggest that the anti-TRX s inhibites TRX h gene expression,and leads to the decreases of the free -SH content in various proteins and increases the contents of albumins,gliadins,globulins and glutelins,resulting in slower germination in transgenic wheat.