生殖生长期两优培九功能叶光反应特性

在野外条件下，利用荧光动力学分析和生理生化研究技术，对生殖生长期超高产杂交稻两优培九和大面积推广稻汕优63功能叶原初反应、电子传递和光合磷酸化水平进行系统研究，比较光反应特性。结果表明: (1) 与汕优63相比，两优培九功能叶叶绿素含量高22.90%，光合功能期长约50%，叶绿素a/b比值高36.78%；(2) 光能吸收与分配方面，两优培九功能叶单位叶面积吸收的光能不占优势，但保持高吸收的稳定期长，有活性的反应中心数量多，热耗散的能量比例较少，进入电子传递链的能量高；(3) 荧光参数分析发现两优培九PSII供体侧、受体侧和反应中心性能优良，光能吸收、传递和转化为电能效率较高；(4) 叶绿体放氧活性、电子传递链和光合磷酸化活性均显著高于汕优63，表明两优培九电能转化为活跃化学能能力强。

Light Reaction Characteristics in Functional Leaves of Liangyoupeijiu in the Reproductive Growth Stage

The chloroplast light reaction characteristics were compared between super-high-yield hybrid rice Liangyoupeijiu and traditional hybrid rice Shanyou 63 intending to provide theoretical insights into physiological basis for high yield. Using fluorescence dynamics analysis and physiological and biochemical research techniques, in the field we systematically studied the primary response, electron transport chain and photophosphorylation during the reproductive period. The results showed that: (1) as compared to Shanyou 63, chlorophyll content in functional leaves of Liangyoupeijiu was 22.90% higher, it had a relatively longer photosynthetic function duration and chla/chlb ratio was 36.78% higher; (2) there was no significant difference in light absorption per unit leaf area of functional leaves, but Liangyoupeijiu maintained a high light energy absorption capacity and long stability period , the number of active reaction centers was more, the energy of heat dissipation was relatively lower, the energy transferred into the electron transport chain was higher; (3) fluorescence analysis showed that structures and status of the body side, the receptor side and the reaction center in PSII performed better than those of Shanyou 63, indicating that Liangyoupeijiu had a higher efficiency in the transforming light energy into electric energy; (4) in addition, oxygen evolution of the chloroplast, activities of electron transport chain and photophosphorylation were significantly higher, indicating that Liangyoupeijiu possessed some advantages in the energy convertsion from electric energy to active chemical energy. With higher light energy absorption, transmission and conversion efficiency, Liangyoupeijiu established a physicological basis of super-high-yield.