玉米自交系高频率再生因素研究

以玉米自交系幼胚为外植体，就不同基因型、不同激素对其愈伤组织诱导和植株再生的影响进行了研究，并建立了玉米自交系高频率再生系统。方差分析表明：玉米自交系的高频率再生受基因型、激素以及二者间的互作影响。其中基因型是最重要的影响因素。同时，再生植株的分化还受到诱愈培养基、分化培养基、诱愈培养基与分化培养基

Factors Affecting the Frequencies of Callus Induction and Plantlet Regeneration in Maize Immature Embryo Culture

Four maize genotypes were used to study the factors affecting callus induction and subsequent plantlet regeneration in maize immature embryo culture. Highly efficient plant regeneration systems were established for the tested inbreds. It was revealed that the regeneration of maize inbreds was affected by genotype, regeneration medium, and the interaction between genotype and regeneration media. Genotype was the most important factor compared to callus inducing medium,regeneration medium and the interaction between callus induction and regeneration media. The optimum callus inducing medium for immature embryos of maize was N 6 basics 2 mg/L 2,4 D 10 mg/L AgNO 3 300 mg/L proline 500 mg/L hydrolytic casein 20 g/L sucrose 10 g/ L mannitol. The optimum medium for plantlet regeneration was MS 1 mg/L 6 BA 0.1 mg/L IAA 500 mg/L hydrolytic casein 30 g/L sucrose.