| 巴西橡胶树自根幼态无性系的试管微繁 |
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| 引用本文： | 陈雄庭,王泽云,吴胡蝶,谢玉萍,郑文如.巴西橡胶树自根幼态无性系的试管微繁[J].作物学报,1998,24(2):225-230. |
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| 作者姓名： | 陈雄庭 王泽云 吴胡蝶 谢玉萍 郑文如 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术国家重点实验室,海南儋州,571737 |
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| 基金项目： | “八五”农业部重点攻关计划资助项目 |
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| 摘 要： | 带有腋芽的橡胶花药苗切段接种在MS 6—BA2mg/L的培养基上,30～40天新芽可长至3～5cm高,并可切割再次增殖;切取3～5cm高带叶片的增殖芽作为插条,基部经50～100mg/L IBA预处理再插到生根培养基上,生根率达95％;诱发的根系与茎杆之间愈合良好,输导组织连接紧密,生根植株移栽成活率达85％以上。
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| 关 键 词： | 巴西橡胶 试管微繁 |
| 收稿时间： | 1996-08-07 |
| 修稿时间： | 1997-05-21 |
In Vitro Micropropagation of Juvenile Selfroot-clones of Hevea brasiliensis |
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| Chen Xiongting Wang Zeyun Wu Hudie Xie Yuping Zheng Wenru.In Vitro Micropropagation of Juvenile Selfroot-clones of Hevea brasiliensis[J].Acta Agronomica Sinica,1998,24(2):225-230. |
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| Authors: | Chen Xiongting Wang Zeyun Wu Hudie Xie Yuping Zheng Wenru |
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| Institution: | National Key Biotechnology Laboratory for Tropical Crops,CATAS,Danzhou,Hainan,571737 |
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| Abstract: | Anther plantlets of Hevea brasiliensis were cut into segments each with one bud
at least, and were inoculated on the MS medium supplemented with 6-BA 2 mg/L. The buds
grew into shoots of 3 to 5 cm high in 30 to 40 days and were cut again into segments to multiply.
The shoots of 3 to 5 cm high were cut off as cuttings and pretreated at the base with IBA 50~
100 mg/L and cultured on the rooting medium. 95% of cuttings formed roots. The root formed a
good coalescence with the stem and the vascular bundles were unionized compactly. The rooted
cuttings were transplanted into soil with a survival ratio of more than 85%. |
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| Keywords: | Hevea brasiliensis Micropropagation in vitro |
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