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~(12)C~(6+)离子束辐照糜子诱变突变群体的构建与SSR分析
引用本文:刘天鹏,董孔军,董喜存,何继红,刘敏轩,任瑞玉,张磊,杨天育.~(12)C~(6+)离子束辐照糜子诱变突变群体的构建与SSR分析[J].作物学报,2018,44(1):144-156.
作者姓名:刘天鹏  董孔军  董喜存  何继红  刘敏轩  任瑞玉  张磊  杨天育
作者单位:甘肃省农业科学院作物研究所;中国科学院近代物理研究所;中国农业科学院作物科学研究所;甘肃农业大学生命科学技术学院;
基金项目:The work was supported by the Gansu Academy of Agricultural Sciences Funds for Youth and the China Agriculture Research System (CARS-07-12.5-A5).
摘    要:为构建物理诱变糜子突变体库,采用50、100、150、200和250 Gy剂量碳离子束(~(12)C~(6+))辐照陇糜7号和晋黍9号种子,结合混合系谱法和系统聚类分别构建2个含52和79个株系的M5寡表型突变群体。田间试验结果显示,诱变M1出苗率随剂量增大明显降低,陇糜7号M1半致死剂量为150 Gy,晋黍9号M1半致死剂量为100 Gy,且100、150 Gy诱变下M4变异最多。不同诱变剂量下M5、M6表型稳定且株高、产量性状、成株色及粒色均表现出明显遗传差异。从2个M6群体中分别选择9和11个代表性株系,利用多态性SSR引物进行分子验证,与亲本相比,6对SSR引物在陇糜7号的9个株系中位点变异基因型数为1~2,在晋黍9号的11个株系中位点变异基因型数为1~4,突变群体存在丰富的遗传多样性。

关 键 词:碳(12C6+)离子  物理诱变  突变体  谱系  SSR标记
收稿时间:2017-03-24

Pedigree Construction and SSR Analysis of Broomcorn Millet Mutant by 12C6+ Ion Beam Irradiation
LIU Tian-Peng,DONG Kong-Jun,DONG Xi-Cun,HE Ji-Hong,LIU Min-Xuan,REN Rui-Yu,ZHANG Lei,YANG Tian-Yu.Pedigree Construction and SSR Analysis of Broomcorn Millet Mutant by 12C6+ Ion Beam Irradiation[J].Acta Agronomica Sinica,2018,44(1):144-156.
Authors:LIU Tian-Peng  DONG Kong-Jun  DONG Xi-Cun  HE Ji-Hong  LIU Min-Xuan  REN Rui-Yu  ZHANG Lei  YANG Tian-Yu
Institution:1.Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;4.Life Sciences and Technology College, Gansu Agricultural University, Lanzhou 730070, China
Abstract:To construct mutant library of physical mutation in broomcorn millet, applied five doses of 12C6+ ion beam at 50, 100, 150, 200, and 250 Gy to irradiate seed of Longmi 7 and Jinshu 9, and constructed two populations (M5) consisting of 52 and 79 lines respectively with mixed pedigree method and system cluster. The field text showed that the emergence rate of M1 decreased significantly with increasing 12C6+ dose, half lethal dose for M1 from Longmi 7 and Jinshu 9 were 150 Gy and 100 Gy. M4 at 100, 150 Gy produce a most abundant mutants. M5 and M6 Phenotypes were more stable than M4, and plant height, yield traits, plant color and seed color showed significant difference. Nine M6 lines from Longmi 7 and 11 M6 lines from Jinshu 9 were detected with six pairs of SSR primer, compared with the parents, loci variant genotype number were 1-2 and 1-4, respectively, showing abundant genetic diversity in mutant populations.
Keywords:12C6+ ion  Physical irradiation  Mutant populations  Molecular marker
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