小麦籽粒硬度基因型鉴定及其与吹泡仪和混合仪参数关系分析

以我国黄淮麦区35份小麦高代新品系为材料，通过测定其籽粒硬度表型、puroindoline基因型及其吹泡仪和混合仪参数，分析了籽粒硬度表型和基因型与吹泡仪和混合仪参数间的关系。结果发现，SKCS籽粒硬度与混合仪参数中的C1、C2、C3、C4值、幅度、吸水率、C3-C2值以及吹泡仪参数的P值、L值、G值和P/L值之间均极显著相关(P < 0.01)。方差分析表明，puroindoline基因对大多数吹泡仪和混合仪参数的影响极显著(P < 0.01)。其中，Pina-D1b类型小麦品系的P值、W值和P/L值均显著高于野生型和Pinb-D1b类型，但野生型小麦品系的L值、G值和Ie值则均显著高于Pinb-D1b和Pina-D1b类型。另外，2种硬质基因型(Pinb-D1b和Pina-D1b)小麦品系的C1值、C2值、C2-C1值、幅度和吸水率均显著高于野生型，而野生型小麦品系的C3值、C4值和C5值则显著高于2种硬质基因型。

Association of Wheat Kernel Hardness and Puroindoline Genes with Alveograph and Mixolab Parameters

A total of 35 wheat advanced lines with three replications from the Yellow and Huai Valley wheat region of China were identified for SKCS(single kernel characterization system)hardness index and puroindoline alleles as well as parameters of Alveograph and Mixolab.The results indicated that SKCS hardness index possessed highly significant correlations(P < 0.01)with Mixolab parameters of C1,C2,C3,C4,range of torque,water absorption,and C3-C2 and Alveograph parameters of P,L,G,and P/L.Most of the Alveograph and Mixolab parameters were significantly affected(P < 0.01)by puroindoline genes.For Alveograph parameters,the averages of P,W,and P/L were significantly higher(P < 0.05)in the Pina-D1b lines than in the wild type and the Pinb-D1b lines,but the averages of L,G,and Ie were significantly higher(P < 0.05)in the wild type than in both Pina-D1b and Pinb-D1b lines.For Mixolab parameters,the averages of C1,C2,C2-C1,range of torque,and water absorption were significantly higher(P < 0.05)in both Pinb-D1b and Pina-D1b lines than in the wild type,whereas the averages of C3,C4,and C5 were significantly higher(P < 0.05)in the wild type than in the Pinb-D1b and Pina-D1b lines.