甘蓝自交不亲和信号传导中SRK底物ARC1蛋白编码序列的克隆与分析

以甘蓝自交不亲和系20010197的植株为材料，提取幼苗中的gDNA及花蕾期的柱头、子房、花瓣、叶片、根的mRNA，并对mRNA进行反转录合成cDNA，然后以gDNA与cDNA为模板扩增ARC1蛋白编码序列。结果表明只在gDNA与花期柱头mRNA的反转录cDNA中扩增出产物，而在其他组织中无任何产物。这可能说明甘蓝ARC1蛋白编码序列是在柱头特异性表

Cloning and Characterization of Encoding Sequence of SRK-Binding Protein ARC1 from Brassica oleracea L.in Self-incompatibility Signaling Process

Cloning by PCR and sequencing of ARC1Boler gene from genomic DNA and stigma cDNA in Brassica oleracea L. of self-incompatibility are immensely important. It helps to understand of ARC1 gene mediating in self-incompatibility signaling process. In this paper, ARC1Boler gene was amplified and sequenced by PCR with template genomic DNA and cDNA from roots, leaves, petals, stigma and ovaries during bud stage. The major results are as follows: ARC1Boler gene was steadily amplified from genomic DNA and stigma cDNA, but there was no PCR products using cDNA of roots, leaves, petals and ovaries as templates, which suggested that ARC1Boler gene was expressed only in the stigma. ARC1Boler gene showed 94% identity to the sequence of ARC1 gene of Brassica napus L. There was only a 1 983 bp ORF in ARC1Boler gene which encodes 661 amino acids. The sequence of its amino acids showed 85% and 52% identity to those of ARC1Bnap gene and ARC1At gene respectively. In three proteins, there were two structures, Ubox and Arm Repeats. Ubox was possibly the domain of dimeration of ARC1 protein. In the C-terminal of ARC1Boler protein and ARC1Bnap protein, there were five arm repeats, while only one in ARC1At protein. This difference may potentially determine the discrepancy of function of ARC1Boler, ARC1Bnap and ARC1At. In ARC1Boler protein and ARC1Bnap protein, the arm repeats of C-terminal are conceivably conservative domain.