基于HPLC-RID的花生籽仁可溶性糖含量检测方法的建立

食用型花生是我国乃至世界范围内花生育种的重要方向之一,但是花生遗传改良中缺乏与食用品质相关的可溶性糖含量的快速检测方法,限制了食用花生育种进展。本研究建立了80%乙醇和水浴快速提取花生籽仁可溶性糖的方法,该提取方法与国标法相比,简化了样品前期处理步骤,加快了提取进度。通过准确性和重复性试验对该方法的验证表明,该方法的重复性较好,而且准确有效。以20份花生品种为材料,利用高效液相-示差折光法对该方法提取的样品和国标法提取的样品进行检测发现,成熟花生籽仁中的可溶性糖主要是蔗糖,葡萄糖和果糖很少,2种方法测定的结果差异不显著。利用本研究建立的方法检测20份花生品种的结果显示,蔗糖含量最低为16.19 mg g^-1,最高为83.81 mg g^-1,平均为30.41 mg g^-1。利用国标法检测的结果显示,蔗糖含量最低为15.60 mg g^-1,最高为81.38 mg g^-1,平均为30.20 mg g^-1。这些检测结果,一方面进一步验证了所建立方法的实用性,另一方面也表明这些花生品种中的蔗糖含量差异较大。

Establishment of HPLC-RID method for the determination of soluble sugars in peanut seed

Edible peanut is one of the important directions of peanut breeding in China and even in the world.However,the lack of efficient detection method of soluble sugar content related to edible quality in peanut kernel limits the progress of edible peanut breeding.In this study,a method of extracting soluble sugar from peanut kernel by 80%ethanol and water bath was established.The method simplified the pre-treatment steps of samples and speeded up the extraction progress compared with the GB method.The accuracy and repeatability tests showed that this method had high repeatability accuracy and effectiveness.Then,the samples extracted by this method and GB method were both tested by HPLC-RID,it was found that the soluble sugar in mature peanut kernel was mainly sucrose,with little glucose and fructose in 20 peanut varieties.There were no significant differences between these two methods.The results of 20 peanut varieties using the method and GB method showed that the sucrose content ranged from 16.19 mg g^-1 to 83.81 mg g^-1,15.60 mg g^-1 to 81.38 mg g^-1,and with an average of 30.41 mg g^-1,30.20 mg g^-1.In summary,the results not only further verified the practicability of the method established in this study,but also showed that there was a huge difference in the sucrose content between these peanut varieties.