利用90k芯片技术进行小麦穗部性状QTL定位

小麦穗部性状与产量密切相关,挖掘穗部性状基因及其关联分子标记具有重要意义。本研究以周8425B?小偃81衍生的RIL群体(F8)为材料,利用90k芯片标记构建的高密度遗传图谱对3个环境下的穗长、小穗数、不育小穗数、穗粒数、千粒重进行QTL定位。共检测到19条染色体上的71个QTL,变异解释率(PVE)范围为2.10%~45.25%,其中37个位点为主效QTL(PVE10%)。QSl.nafu-6A.2(穗长)、QSl.nafu-7A(穗长)、QSsn.nafu-2A.1(不育小穗数)、QSsn.nafu-2D(不育小穗数)和QGns.nafu-2B(穗粒数)在多个环境中被检测到,且LOD10,PVE20%。位于同一个基因簇中的QSl.nafu-6A.2(穗长)、QGns.nafu-6A(穗粒数)和QTgw.nafu-6A(千粒重)在多个环境中被检测到,且与已报道的相关位点位置相同或相近,在分子标记辅助育种中具有较大参考价值。

QTL Mapping for Spike Traits of Wheat Using 90k Chip Technology

Spike traits are important to grain yield in wheat. Molecular markers associated with genes/QTLs controlling spike traits are highly valuable to marker-assisted breeding. A recombinant inbred line (F8) population derived from Zhou 8425B ? Xiaoyan 81 were evaluated in three environments, and QTLs for spike length, spikelet number per spike, sterile spikelet number, grain number per spike and thousand-grain weigh were mapped into a high-density genetic map built by 90k chip. A total of 71 QTLs were located on 19 chromosomes, and the phenotype variation explained (PVE) by a single locus ranged from 2.10% to 45.25%. Thirty-seven loci were considered as main-effect QTLs owing to the PVE larger than 10%. QTLs QSl.nafu-6A.2 for spike length, QSl.nafu-7A for spike length, QSsn.nafu-2A.1 for sterile spikelet number, QSsn.nafu-2D for sterile spikelet number and QGns.nafu-2B for grain number per spike were identified repeatedly in different environments with the LOD value higher than 10 and PVE larger than 20%. QSl.nafu-6A.2 for spike length, QGns.nafu-6A for grain number per spike and QTgw.nafu-6A for thousand-grain weight were mapped in a cluster on chromosome 6A and might be applicable in marker-assisted selection because they have been detected in multiple environments and close to the loci reported.