| 应用自动荧光测序系统分析不同水稻基因型SSLPs |
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| 引用本文： | 李莉,杨剑波,D.J.Mackill,P.M.Colowit.应用自动荧光测序系统分析不同水稻基因型SSLPs[J].作物学报,2000,26(5):565-569. |
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| 作者姓名： | 李莉 杨剑波 D.J.Mackill P.M.Colowit |
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| 作者单位： | 安徽省农业科学院水稻遗传育种农业部重点开放实验室， 安徽合肥， 230031 |
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| 摘 要： | 本研究使用30对微卫星引物， 对14个不同类型及来源的水稻基因型材料进行SSR片段长度的多态性分析， 并运用自动荧光测序系统精确检测SSLP片段的长度， 结合GeneScan2.1和Genotype2.0分析软件进行数据输出和图象处理， 得到了多态性丰富的微卫星标记。 可以利用这些标记区别和鉴定不同类型和来源的水稻种质材料， 并进行亲源
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| 关 键 词： | 自动荧光测序 水稻 简单序列重复 多态性分析 |
| 收稿时间： | 1999-09-14 |
| 修稿时间： | 1999-12-05 |
Application of Automated Fluorescent-labelled System for SSLPs Analysis of Different Rice Genotypes |
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| D.J.Mackill,P.M.Colowit.Application of Automated Fluorescent-labelled System for SSLPs Analysis of Different Rice Genotypes[J].Acta Agronomica Sinica,2000,26(5):565-569. |
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| Authors: | DJMackill PMColowit |
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| Institution: | Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry, Anhui Academy of Agricultural Sciences, Hefei, 230031 |
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| Abstract: | By using 30 pairs of microsatellite DNA primers, SSLPs (simple sequence length polymorphisms) of 14 rice genotypes were analyzed with the automated fluorescent-labelled system supported by GeneScan2.1 and Genotype 2.0 software. Microsatellite DNA markers with aboudant polymorphism were gained, which |
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| Keywords: | Automated fluorescent-labelled Rice Simple Sequence Repeats Polymorphism |
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