苎麻茎皮环状RNA表达谱分析

苎麻Boehmeria nivea(L.)Gaud.]是我国特色的天然纤维作物,其纤维具有拉伸力强、纤维束长等特点。解析苎麻纤维发育调控机制,对实现纤维产量和品质性状遗传改良具有重要意义。本研究基于高通量测序技术开展了苎麻茎顶部和中部的茎皮组织环状RNA(circRNA)分析,在2个组织中共鉴定到5268个苎麻circRNA。比较circRNA的表达水平发现,78个circRNA在2个组织中呈现差异表达。因苎麻茎中部韧皮纤维处于生长发育期,而茎顶部韧皮纤维尚未起始生长,推测这78个circRNA是纤维不同发育时期差异表达基因,它们可能参与了苎麻的纤维发育调控。研究结果将为解析circRNA调控苎麻纤维生长发育机制奠定基础。

Characterization of the expression profiling of circRNAs in the barks of stemsin ramie

RamieBoehmeria nivea(L.)Gaud.]is a special natural fiber crops in China,and its fiber has many excellent characteristics,including long strands and well tensile strength.Elucidation of the mechanism for fiber formation will be helpful for the improvement of fiber yield and quality in ramie.In this study,the expressed analysis of circular RNA(circRNA)for the tissues of barks from the top stems and middle stems were performed by Illumina sequencing,respectively.The total of 5268 circRNAs were identified.Among these circRNAs,78 showed differential expression between two examined tissues.Previous cytological observation suggested that the secondary cellular walls(SCWs)of fiber cells from the top of the ramie stems did not initiate growth and those from middle part of the ramie stems were thickening.Therefore,we speculated that these 78 differentially expressed circRNAs were potentially involved in the fiber development in ramie.The results provide an important basis for understanding the role of circRNA in the regulation of fiber development.